A Rapid Colorimetric Method to Visualize Protein Interactions

doi:10.1002/chem.201800401

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	A Rapid Colorimetric Method to Visualize Protein Interactions
	Liu, Bing 1,3; Wang, Zhihao 3; Lan, Ling 4; Yang, Qianfan 5; Zhang, Peipei 1; Shi, Lei 2; Lang, Yunhe 2; Tabib-Salazar, Aline 3; Wigneshweraraj, Sivaramesh 3; Zhang, Jiye 1
刊名	CHEMISTRY-A EUROPEAN JOURNAL
	2018-05-07
卷号	24 期号:26 页码:6727-6731
关键词	Colorirnetry Nmr Spectroscopy Protein-protein Interactions Supramolecular Chemistry
ISSN号	0947-6539
DOI	10.1002/chem.201800401
英文摘要	As key molecules in most biological pathways, proteins physically contact one or more biomolecules in a highly specific manner. Several driving forces (i.e., electrostatic and hydrophobic) facilitate such interactions and a variety of methods have been developed to monitor these processes both in vivo and in vitro. In this work, a new method is reported for the detection of protein interactions by visualizing a color change of a cyanine compound, a supramolecule complex of 3,3-di-(3-sulfopropyl)-4,5,4',5'-dibenzo-9-methyl-thiacarbocyanine triethylammonium salt (MTC). Nuclear magnetic resonance (NMR) studies suggest that the hydrophobic nature of the protein surfaces drives MTC into different types of aggregates with distinct colors. When proteins interact with other biomolecules, the hydrophobic surface of the complex differs, resulting in a shift in the form of MTC aggregation, which results in a color change. As a result, this in vitro method has the potential to become a rapid tool for the confirmation of protein-biomolecule interactions, without the requirements for sophisticated instrumentation or approaches.
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000431492500009
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/42767]
专题	中国科学院化学研究所
通讯作者	Matthews, Steve; Zhang, Xiufeng
作者单位	1.Xi An Jiao Tong Univ, BioBank, Affiliated Hosp 1, Sch Med, Xian 710049, Shaanxi, Peoples R China 2.North China Univ Sci & Technol, Coll Chem Engn, Tangshan 063210, Peoples R China 3.Imperial Coll London, Prof Dr S Matthews Dept Life Sci, London, England 4.Chinese Acad Sci, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Inst Chem, Beijing 100190, Peoples R China 5.Sichuan Univ, Coll Chem, Chengdu 610065, Sichuan, Peoples R China
推荐引用方式 GB/T 7714	Liu, Bing,Wang, Zhihao,Lan, Ling,et al. A Rapid Colorimetric Method to Visualize Protein Interactions[J]. CHEMISTRY-A EUROPEAN JOURNAL,2018,24(26):6727-6731.
APA	Liu, Bing.,Wang, Zhihao.,Lan, Ling.,Yang, Qianfan.,Zhang, Peipei.,...&Zhang, Xiufeng.(2018).A Rapid Colorimetric Method to Visualize Protein Interactions.CHEMISTRY-A EUROPEAN JOURNAL,24(26),6727-6731.
MLA	Liu, Bing,et al."A Rapid Colorimetric Method to Visualize Protein Interactions".CHEMISTRY-A EUROPEAN JOURNAL 24.26(2018):6727-6731.