Binding of Organometallic Ruthenium Anticancer Complexes to DNA: Thermodynamic Base and Sequence Selectivity

doi:10.3390/ijms19072137

CORC > 化学研究所 > 中国科学院化学研究所

	Binding of Organometallic Ruthenium Anticancer Complexes to DNA: Thermodynamic Base and Sequence Selectivity
	Liu, Suyan 1,2; Liang, Aihua 1; Wu, Kui 2,3; Zeng, Wenjuan 2,4; Luo, Qun 2,4; Wang, Fuyi 2,4
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2018-07-01
卷号	19 期号:7
关键词	Organometallic Ruthenium Complexes Anticancer Oligodeoxynucleotide Base/sequence Selectivity Thermodynamics Lc-ms
ISSN号	1422-0067
DOI	10.3390/ijms19072137
英文摘要	Organometallic ruthenium(II) complexes [(eta(6)-arene) Ru(en) Cl][PF6] (arene = benzene (1), p-cymene (2), indane (3), and biphenyl (4); en = ethylenediamine) are promising anticancer drug candidates both in vitro and in vivo. In this paper, the interactions between ruthenium(II) complexes and 15-mer single- and double-stranded oligodeoxynucleotides (ODNs) were thermodynamically investigated using high performance liquid chromatography (HPLC) and electrospray ionization mass spectroscopy (ESI-MS). All of the complexes bind preferentially to G(8) on the single strand 5'-CTCTCTT7G(8)T(9)CTTCTC-3' (I), with complex 4 containing the most hydrophobic ligand as the most reactive one. To the analogs of I (changing T7 and/or T-9 to A and/or C), complex 4 shows a decreasing affinity to the G(8) site in the following order:-AG(8)T-(K: 5.74 x 10(4) M-1) >-CG(8)C-> -TG(8)A->-AG(8)A->-AG(8)C->-TG(8)T-(I)approximate to-CG(8)A-(K: 2.81 x 10(4) M-1). In the complementary strand of I, the G bases in the middle region are favored for ruthenation over guanine (G) bases in the end of oligodeoxynucleotides (ODNs). These results indicate that both the flanking bases (or base sequences) and the arene ligands play important roles in determining the binding preference, and the base-and sequence-selectivity, of ruthenium complex in binding to the ODNs.
语种	英语
出版者	MDPI
WOS记录号	WOS:000442807400316
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/42357]
专题	中国科学院化学研究所
通讯作者	Wu, Kui; Wang, Fuyi
作者单位	1.China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci,Natl Ctr Mass Spectromet, CAS Res Educ Ctr Excellence Mol Sci,Inst Chem, Beijing 100190, Peoples R China 3.Wuhan Univ Sci & Technol, Sch Chem & Chem Engn, Wuhan 430081, Hubei, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Suyan,Liang, Aihua,Wu, Kui,et al. Binding of Organometallic Ruthenium Anticancer Complexes to DNA: Thermodynamic Base and Sequence Selectivity[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2018,19(7).
APA	Liu, Suyan,Liang, Aihua,Wu, Kui,Zeng, Wenjuan,Luo, Qun,&Wang, Fuyi.(2018).Binding of Organometallic Ruthenium Anticancer Complexes to DNA: Thermodynamic Base and Sequence Selectivity.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,19(7).
MLA	Liu, Suyan,et al."Binding of Organometallic Ruthenium Anticancer Complexes to DNA: Thermodynamic Base and Sequence Selectivity".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 19.7(2018).