A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging

	A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging
	Guo, Lan ; Yang, Daoshan ; Xia, Lian ; Qu, Fei ; Dou, Yanan ; Qu, Fengli ; Kong, Rongmei ; You, Jinmao
刊名	NEW JOURNAL OF CHEMISTRY
	2017-12-21
英文摘要	In this study, a coumarin-based probe (probe 1) bearing a maleimide group was used to rapidly and selectively detect thiols. After inducing thiols, this probe presented a significant fluorescence enhancement (>22-fold), whereas other amino acids that had no sulfhydryl group just generated slight changes. The detection limits were determined to be as low as 7.41 nM for Cys, 6.06 nM for GSH, and 7.73 nM for Hcy based on S/N = 3. Furthermore, this probe possessed excellent water solubility and successfully detected thiols in PBS (pH 7.4, 99.5%). More importantly, the practical application of probe 1 for the selective detection of thiols was successfully demonstrated in living T24 cells.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/13284]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Guo, Lan,Yang, Daoshan,Xia, Lian,et al. A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging[J]. NEW JOURNAL OF CHEMISTRY,2017.
APA	Guo, Lan.,Yang, Daoshan.,Xia, Lian.,Qu, Fei.,Dou, Yanan.,...&You, Jinmao.(2017).A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging.NEW JOURNAL OF CHEMISTRY.
MLA	Guo, Lan,et al."A highly water-soluble, sensitive, coumarin-based fluorescent probe for detecting thiols, and its application in bioimaging".NEW JOURNAL OF CHEMISTRY (2017).