Hadal water sampling by in situ microbial filtration and fixation (ISMIFF) apparatus | |
He, Li-Sheng2; Wang, Yong2; Gao, Zhao-Ming2; Li, Jun2; Cui, Guo-Jie1,2; Li, Wen-Li1,2; Chen, Jun1,2; Xin, Yong-Zhi2; Cai, Du-Si2; Zhang, Ai-Qun2 | |
刊名 | DEEP-SEA RESEARCH PART I-OCEANOGRAPHIC RESEARCH PAPERS |
2019-02-01 | |
卷号 | 144期号:1页码:132-137 |
关键词 | Hadal Trench In Situ samplIng Ismiff Microbial Community |
ISSN号 | 0967-0637 |
DOI | 10.1016/j.dsr.2019.01.009 |
英文摘要 | The deepest point of the oceans is over 10 km depth and is characterized with extremely high hydrostatic pressure. Water sampling from the hadal zone for microbiological studies is therefore difficult. In this study, we demonstrate the application of an in situ microbial filtration and fixation (ISMIFF) apparatus in sampling work from the Challenger Deep. A total of 2300 L hadal waters were filtered by the ISMIFF equipped on a hadal lander during seven deployments. The microbial communities in the hadal samples ( > 6000 m) collected by the ISMIFF were composed of more heterotrophic Marinimicrobia and less photoautotrophic Prochlorococcus and SAR11 bacteria, compared with those by Niskin bottles. Such a difference in the community structure was not observed in the samples collected by the two methods from 5000-m depth, indicating an effect of greater depths on sample quality. Our results indicate that the ISMIFF or similar filtration apparatuses are indispensable for hadal water sampling since using Niskin bottles is probably difficult to obtain pristine samples from the hadal zone. Application of the ISMIFF will pave the way to meta-omics studies on the hadal microbes. |
资助项目 | National Key Research and Development Program of China[2016YFC0302500] ; Strategic Priority Research Program B of Chinese Academy of Sciences[XDB06010201] ; Strategic Priority Research Program B of Chinese Academy of Sciences[XDB06010103] ; Strategic Priority Research Program B of Chinese Academy of Sciences[XDB06040100] ; National Science Foundation of China[41476104] ; National Science Foundation of China[31460001] |
WOS关键词 | Ocean ; Sea ; Bacteria ; Trench |
WOS研究方向 | Oceanography |
语种 | 英语 |
出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
WOS记录号 | WOS:000460831000011 |
资助机构 | National Key Research and Development Program of China ; Strategic Priority Research Program B of Chinese Academy of Sciences ; National Science Foundation of China |
内容类型 | 期刊论文 |
版本 | 出版稿 |
源URL | [http://ir.idsse.ac.cn/handle/183446/6665] |
专题 | 深海科学研究部_深海生物学研究室_海洋微生物宏基因组与分子进化研究组 深海工程技术部_深海探测技术研究室 |
通讯作者 | Wang, Yong |
作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Deep Sea Sci & Engn, 28 Luhuitou Rd, Sanya, Hainan, Peoples R China |
推荐引用方式 GB/T 7714 | He, Li-Sheng,Wang, Yong,Gao, Zhao-Ming,et al. Hadal water sampling by in situ microbial filtration and fixation (ISMIFF) apparatus[J]. DEEP-SEA RESEARCH PART I-OCEANOGRAPHIC RESEARCH PAPERS,2019,144(1):132-137. |
APA | He, Li-Sheng.,Wang, Yong.,Gao, Zhao-Ming.,Li, Jun.,Cui, Guo-Jie.,...&Zhang, Ai-Qun.(2019).Hadal water sampling by in situ microbial filtration and fixation (ISMIFF) apparatus.DEEP-SEA RESEARCH PART I-OCEANOGRAPHIC RESEARCH PAPERS,144(1),132-137. |
MLA | He, Li-Sheng,et al."Hadal water sampling by in situ microbial filtration and fixation (ISMIFF) apparatus".DEEP-SEA RESEARCH PART I-OCEANOGRAPHIC RESEARCH PAPERS 144.1(2019):132-137. |
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