The neuropeptides of Asian freshwater clam (Corbicula fluminea) as new molecular biomarker basing on the responses of organophosphate chemicals exposure

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院饮用水科学与技术重点实验室

	The neuropeptides of Asian freshwater clam (Corbicula fluminea) as new molecular biomarker basing on the responses of organophosphate chemicals exposure
	Zha, Jinmiao; Yuan, Lilai; Chen, Huihui; Hong, Xiangsheng; Wang, Qi
刊名	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
	2018-09-30
卷号	160 页码:52-59
关键词	TBOEP TBP CCK Conopressin FFamide MRNA expression
ISSN号	0147-6513
文献子类	Article
英文摘要	In the present study, to discover new biomarker of Asian freshwater clam (Corbicula fiuminea) to assess impact of environmental pollutions, cholecystokinin (CCK), conopressin, and Neuropeptide FF (FFamide) in C. fluminea were selected as potent biomarkers. Therefore, their full-length cDNAs were cloned and characterized to investigate the molecular characteristics and expression patterns of neuropeptides in C. fiuminea. According to the sequence analysis, CCK, conopressin, and FFamide encoded proteins of 173, 152, and 90 amino acids, respectively. Moreover, the multiple sequence alignment revealed that the bioactive regions of these neuropeptides were well conserved among different invertebrates. In addition, under basal conditions, CCK, conopressin and FFamide mRNA were mainly expressed in the visceral mass, whereas the FFamide mRNA was rarely detected in the foot and mantle. Exposure to 20 and 200 mu g/L Tris (2-butoxyethyl) phosphate (TBOEP) and tri-butyl-phosphate (TBP) exposure significantly up-regulated the expression of the CCK and FFamide mRNAs in the visceral mass (p < 0.05), whereas no significant changes in conopressin mRNA levels were observed in response to any treatment. Therefore, CCK and FFamide of C. fluminea neuropeptides are feasible new biomarkers for screening and assessing responses to organophosphate chemicals.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/41424]
专题	生态环境研究中心_中国科学院饮用水科学与技术重点实验室
推荐引用方式 GB/T 7714	Zha, Jinmiao,Yuan, Lilai,Chen, Huihui,et al. The neuropeptides of Asian freshwater clam (Corbicula fluminea) as new molecular biomarker basing on the responses of organophosphate chemicals exposure[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2018,160:52-59.
APA	Zha, Jinmiao,Yuan, Lilai,Chen, Huihui,Hong, Xiangsheng,&Wang, Qi.(2018).The neuropeptides of Asian freshwater clam (Corbicula fluminea) as new molecular biomarker basing on the responses of organophosphate chemicals exposure.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,160,52-59.
MLA	Zha, Jinmiao,et al."The neuropeptides of Asian freshwater clam (Corbicula fluminea) as new molecular biomarker basing on the responses of organophosphate chemicals exposure".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 160(2018):52-59.