Tracking antibiotic resistome during wastewater treatment using high throughput quantitative PCR

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	Tracking antibiotic resistome during wastewater treatment using high throughput quantitative PCR
	Zhao, Yi; Zhu, Yong-Guan; Zhang, Tong; Gillings, Michael R.; Chen, Hong; Cui, Li; Chen, Qing-Lin; Ouyang, Wei-Ying; Li, Bing; Su, Jian-Qiang
刊名	ENVIRONMENT INTERNATIONAL
	2018-08-01
卷号	117 页码:146-153
关键词	Antibiotic resistance genes Human pathogens Horizontal gene transfer Human health Urban environment
ISSN号	0160-4120
文献子类	Article
英文摘要	Wastewater treatment plants (WWTPs) contain diverse antibiotic resistance genes (ARGs), and thus are considered as a major pathway for the dissemination of these genes into the environments. However, comprehensive evaluations of ARGs dynamic during wastewater treatment process lack extensive investigations on a broad spectrum of ARGs. Here, we investigated the dynamics of ARGs and bacterial community structures in 114 samples from eleven Chinese WWTPs using high-throughput quantitative PCR and 16S rRNA-based Illumina sequencing analysis. Significant shift of ARGs profiles was observed and wastewater treatment process could significantly reduce the abundance and diversity of ARGs, with the removal of ARGs concentration by 1-2 orders of magnitude. Whereas, a considerable number of ARGs were detected and enriched in effluents compared with influents. In particular, seven ARGs mainly conferring resistance to beta-lactams and aminoglycosides and three mobile genetic elements persisted in all WWTPs samples after wastewater treatment. ARGs profiles varied with wastewater treatment processes, seasons and regions. This study tracked the footprint of ARGs during wastewater treatment process, which would support the assessment on the spread of ARGs from WWTPs and provide data for identifying management options to improve ARG mitigation in WWTPs.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/41232]
专题	生态环境研究中心_土壤环境科学实验室
推荐引用方式 GB/T 7714	Zhao, Yi,Zhu, Yong-Guan,Zhang, Tong,et al. Tracking antibiotic resistome during wastewater treatment using high throughput quantitative PCR[J]. ENVIRONMENT INTERNATIONAL,2018,117:146-153.
APA	Zhao, Yi.,Zhu, Yong-Guan.,Zhang, Tong.,Gillings, Michael R..,Chen, Hong.,...&An, Xin-Li.(2018).Tracking antibiotic resistome during wastewater treatment using high throughput quantitative PCR.ENVIRONMENT INTERNATIONAL,117,146-153.
MLA	Zhao, Yi,et al."Tracking antibiotic resistome during wastewater treatment using high throughput quantitative PCR".ENVIRONMENT INTERNATIONAL 117(2018):146-153.