Microbial community response and SDS-PAGE reveal possible mechanism of waste activated sludge acidification enhanced by microaeration coupled thermophilic pretreatment

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院环境生物技术重点实验室

	Microbial community response and SDS-PAGE reveal possible mechanism of waste activated sludge acidification enhanced by microaeration coupled thermophilic pretreatment
	Cai, Wei-Wei; Hou, Ya-Nan; Yang, Chunxue; Zhou, Aijuan; Liu, Wenzong; Liu, Chong; Zhou, Jizhong; Wang, Ai-Jie
刊名	PROCESS BIOCHEMISTRY
	2018
卷号	64 页码:1-8
关键词	Waste activated sludge (WAS) Pretreatment Micro-aeration Thermophilic Short-chain fatty acids (SCFAs)
ISSN号	1359-5113
文献子类	Article
英文摘要	Aiming to strengthen the performance of waste activated sludge digestion, pretreatment is a prerequisite for keeping operations within an industrially acceptable time-frame. In this study, the performance of microaeration coupled with thermophilic (MT) pretreatment on waste activated sludge solubilization and acidification was investigated. The results showed that the maximum soluble organics concentrations, including soluble proteins and carbohydrates, reached 2290 mg COD/L in 24 h when the ventilation rate and temperature were 0.05 vvm and 70 degrees C. SDS-polyacrylamide gel electrophoresis (SDS-PAGE) test on pure-culture (E.coli) and scanning electron microscopy analysis indicated that MT pretreatment effectively destroyed microbial cell wall and resulted in an increase in soluble proteins. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis revealed that MT pretreatment reduced the diversity of the bacterial community during pretreatment. Along with the dissolution of a large quantity of organic matter, microbial species such as Lactococcus and Methyloversatilis bloomed at the end of acidification period, which contributed to SCFAs production. This study revealed that MT pretreatment facilitated the WAS hydrolysis efficiency and enhanced SCFAs (3149 mg COD/L with fermentation for 96 h) production, especially for acetic acid (50%) accumulation, which provides a new perspective for the application and recovery of sludge resources.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/41279]
专题	生态环境研究中心_中国科学院环境生物技术重点实验室
推荐引用方式 GB/T 7714	Cai, Wei-Wei,Hou, Ya-Nan,Yang, Chunxue,et al. Microbial community response and SDS-PAGE reveal possible mechanism of waste activated sludge acidification enhanced by microaeration coupled thermophilic pretreatment[J]. PROCESS BIOCHEMISTRY,2018,64:1-8.
APA	Cai, Wei-Wei.,Hou, Ya-Nan.,Yang, Chunxue.,Zhou, Aijuan.,Liu, Wenzong.,...&Wang, Ai-Jie.(2018).Microbial community response and SDS-PAGE reveal possible mechanism of waste activated sludge acidification enhanced by microaeration coupled thermophilic pretreatment.PROCESS BIOCHEMISTRY,64,1-8.
MLA	Cai, Wei-Wei,et al."Microbial community response and SDS-PAGE reveal possible mechanism of waste activated sludge acidification enhanced by microaeration coupled thermophilic pretreatment".PROCESS BIOCHEMISTRY 64(2018):1-8.