Isolation and characterization of biosurfactant-producing and diesel oil degrading Pseudomonas sp CQ2 from Changqing oil field, China

doi:10.1039/c8ra07721e

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋地质与环境重点实验室

	Isolation and characterization of biosurfactant-producing and diesel oil degrading Pseudomonas sp CQ2 from Changqing oil field, China
	Sun, Wuyang 1,2; Cao, Wenrui 3; Jiang, Mingyu 3; Saren, Gaowa 3; Liu, Jiwei 1,2,4; Cao, Jiangfei 4; Ali, Imran 1,2; Yu, Xinke 3; Peng, Changsheng 1,2,4; Naz, Iffat 5,6
刊名	RSC ADVANCES
	2018
卷号	8 期号:69 页码:39710-39720
ISSN号	2046-2069
DOI	10.1039/c8ra07721e
通讯作者	Peng, Changsheng(pcs005@ouc.edu.cn) ; Naz, Iffat(iffatkhattak@yahoo.com)
英文摘要	In the present research investigation, 13 indigenous bacteria (from CQ1 to CQ13) were isolated from soil collected from Changqing oil field of Xi'an, China. Four promising biosurfactant producers (CQ1, CQ2, CQ4, and CQ13) were selected through primary screening among these 13 strains, including via drop collapse and oil-spreading methods. However, only the strain CQ2 showed the best biosurfactant production and was further screened by hemolytic assay, cetyl trimethyl ammonium bromide (CTAB), surface tension and emulsifying activity. The bacterium CQ2 has the ability to produce about 3.015 g L-1 of biosurfactant using glucose as the sole carbon source without any optimization. The produced biosurfactant could greatly reduce surface tension from 72.66 to 24.72 mN m(-1) with a critical micelle concentration (CMC) of 30 mg L-1 and emulsify diesel oil up to 60.1%. The cell-free broth was found to be stable in wide temperature (4-100 degrees C), pH (6-12) and salinity (2-20%) ranges for surface and emulsifying activity. This biosurfactant was preliminarily found to be of a glycolipid nature as evident from thin-layer chromatographic (TLC) and Fourier transform infra-red spectroscopic (FTIR) analyses. Moreover, CQ2 was able to degrade 54.7% of diesel oil, which surprisingly could form a substantial amount of bioflocculants during the degradation process. Furthermore, the 16S rDNA sequence using the Genbank BLAST tool revealed that isolated CQ2 was closely related to species of Pseudomonas genus and, thus, was entitled Pseudomonas sp. CQ2. The results of residual diesel oil contents measured by GC-MS showed that C7-C28 hydrocarbons could be degraded by Pseudomonas sp. CQ2. Thus, these findings revealed that CQ2 could be applied for remediation of diesel oil/petroleum-contaminated waters and soils on a large scale.
资助项目	State Key Laboratory of Environmental Criteria and Risk Assessment[SKLECRA2013FP12] ; Shandong Province Key Research and Development Program[2016GSF115040] ; National Nature Science Foundation of China[41406060]
WOS研究方向	Chemistry
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000452116300054
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/155814]
专题	海洋研究所_海洋地质与环境重点实验室
通讯作者	Peng, Changsheng; Naz, Iffat
作者单位	1.Ocean Univ China, Key Lab Marine Environm Sci & Ecol, Minist Educ, Qingdao 266100, Peoples R China 2.Ocean Univ China, Coll Environm Sci & Engn, Qingdao 266100, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 4.Zhaoqing Univ, Sch Environm & Chem Engn, Zhaoqing 526061, Peoples R China 5.Qassim Univ, Deanship Educ Serv, Dept Biol, Buraydah 51452, Saudi Arabia 6.Quaid i Azam Univ, Dept Microbiol, Islamabad, Pakistan
推荐引用方式 GB/T 7714	Sun, Wuyang,Cao, Wenrui,Jiang, Mingyu,et al. Isolation and characterization of biosurfactant-producing and diesel oil degrading Pseudomonas sp CQ2 from Changqing oil field, China[J]. RSC ADVANCES,2018,8(69):39710-39720.
APA	Sun, Wuyang.,Cao, Wenrui.,Jiang, Mingyu.,Saren, Gaowa.,Liu, Jiwei.,...&Naz, Iffat.(2018).Isolation and characterization of biosurfactant-producing and diesel oil degrading Pseudomonas sp CQ2 from Changqing oil field, China.RSC ADVANCES,8(69),39710-39720.
MLA	Sun, Wuyang,et al."Isolation and characterization of biosurfactant-producing and diesel oil degrading Pseudomonas sp CQ2 from Changqing oil field, China".RSC ADVANCES 8.69(2018):39710-39720.