Phosphoproteomic analyses of kidneys of Atlantic salmon infected with Aeromonas salmonicida

doi:10.1038/s41598-019-38890-3

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Phosphoproteomic analyses of kidneys of Atlantic salmon infected with Aeromonas salmonicida
	Liu, Peng-fei 1,2,3; Du, Yishuai 1,2,3; Meng, Lingjie1,2,3 ; Li, Xian 1,2,3; Yang, Dung 5; Liu, Ying4
刊名	SCIENTIFIC REPORTS
	2019-02-14
卷号	9 页码:12
ISSN号	2045-2322
DOI	10.1038/s41598-019-38890-3
通讯作者	Yang, Dung(yangdongbprc@163.com) ; Liu, Ying(yingliu@dlou.edu.cn)
英文摘要	Aeromonas salmonicida (A. salmonicida) is a pathogenic bacterium that causes furunculosis and poses a significant global risk, particularly in economic activities such as Atlantic salmon (Salmo salar) farming. In a previous study, we identified proteins that are significantly upregulated in kidneys of Atlantic salmon challenged with A. salmonicida. Phosphoproteomic analyses were conducted to further clarify the dynamic changes in protein phosphorylation patterns triggered by bacterial infection. To our knowledge, this is the first study to characterize phosphorylation events in proteins from A. salmonicida-infected Atlantic salmon. Overall, we identified over 5635 phosphorylation sites in 3112 proteins, and 1502 up-regulated and 77 down-regulated proteins quantified as a 1.5-fold or greater change relative to control levels. Based on the combined data from proteomic and motif analyses, we hypothesize that five prospective novel kinases (VRK3, GAK, HCK, PKCd and RSK6) with common functions in inflammatory processes and cellular pathways to regulate apoptosis and the cytoskeleton could serve as potential biomarkers against bacterial propagation in fish. Data from STRING-based functional network analyses indicate that fga is the most central protein. Our collective findings provide new insights into protein phosphorylation patterns, which may serve as effective indicators of A. salmonicida infection in Atlantic salmon.
资助项目	National Key R&D Program of China[2017YFD0701700] ; National Natural Science Foundation of China[31671376] ; Chinese State Key Projects for Basic Research (973 Program)[2015CB910700] ; Chinese State Key Projects for Basic Research (973 Program)[2014CBA02001] ; Beijing Nova Program[Z161100004916148] ; State Key Laboratory of Proteomics[SKLP-O201704] ; State Key Laboratory of Proteomics[SKLP-O201507] ; Innovation project[16CXZ027]
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000458619600100
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/155557]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Yang, Dung; Liu, Ying
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 4.Dalian Ocean Univ, Dalian, Peoples R China 5.Natl Ctr Prot Sci Beijing, State Key Lab Prote, Beijing Proteome Res Ctr, Beijing Inst Life, Beijing 102206, Peoples R China
推荐引用方式 GB/T 7714	Liu, Peng-fei,Du, Yishuai,Meng, Lingjie,et al. Phosphoproteomic analyses of kidneys of Atlantic salmon infected with Aeromonas salmonicida[J]. SCIENTIFIC REPORTS,2019,9:12.
APA	Liu, Peng-fei,Du, Yishuai,Meng, Lingjie,Li, Xian,Yang, Dung,&Liu, Ying.(2019).Phosphoproteomic analyses of kidneys of Atlantic salmon infected with Aeromonas salmonicida.SCIENTIFIC REPORTS,9,12.
MLA	Liu, Peng-fei,et al."Phosphoproteomic analyses of kidneys of Atlantic salmon infected with Aeromonas salmonicida".SCIENTIFIC REPORTS 9(2019):12.