N-doped carbon dots derived from bovine serum albumin and formic acid with one- and two-photon fluorescence for live cell nuclear imaging

doi:10.1016/j.colsurfb.2015.09.008

	N-doped carbon dots derived from bovine serum albumin and formic acid with one- and two-photon fluorescence for live cell nuclear imaging
	Tan, Mingqian 2; Li, Xintong 1,3; Wu, Hao 1; Wang, Beibei 1; Wu, Jing 3
刊名	COLLOIDS AND SURFACES B-BIOINTERFACES
	2015-12-01
卷号	136 页码:141-149
关键词	Carbon Dots Two-photon-induced Fluorescence Bioimaging Nitrogen-doped Nuclear-targeted Imaging
ISSN号	0927-7765
DOI	10.1016/j.colsurfb.2015.09.008
文献子类	Article
英文摘要	Carbon dots with both one- and two-photon fluorescence have drawn great attention for biomedical imaging. Herein, nitrogen-doped carbon dots were facilely developed by one-pot hydrothermal method using bovine serum albumin and formic acid as carbon sources. They are highly water-soluble with strong fluorescence when excited with ultraviolet or near infrared light. The carbon dots have a diameter of similar to 8.32 nm and can emit strong two-photon induced fluorescence upon excitation at 750 nm with a femtosecond laser. X-ray photoelectron spectrometer analysis revealed that the carbon dots contained three components, C, N and 0, corresponding to the peak at 285, 398 and 532 eV, respectively. The Fourier-transform infrared spectroscopy analysis revealed that there are carboxyl and carboxylic groups on the surface, which allowed further linking of functional molecules. pH stability study demonstrated that the carbon dots are able to be used in a wide range of pH values. The fluorescence mechanism is also discussed in this study. Importantly, these carbon dots are biocompatible and highly photostable, which can be directly applied for both one- and two-photon living cell imaging. After proper surface functionalization with TAT peptide, they can be used as fluorescent probes for live cell nuclear-targeted imaging. (C) 2015 Elsevier B.V. All rights reserved.
WOS关键词	ONE-STEP SYNTHESIS ; QUANTUM DOTS ; POLYETHYLENE-GLYCOL ; FACILE SYNTHESIS ; GREEN SYNTHESIS ; PHOTOLUMINESCENCE ; NITROGEN ; LUMINESCENCE
WOS研究方向	Biophysics ; Chemistry ; Materials Science
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000367408100019
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/171514]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Tan, Mingqian
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China 2.Dalian Polytech Univ, Sch Food Sci & Technol, Natl Engn Res Ctr Seafood, Liaoning Prov Key Lab Seafood Sci & Technol, Dalian 116034, Peoples R China 3.Liaoning Normal Univ, Sch Chem & Chem Engn, Dalian 116029, Peoples R China
推荐引用方式 GB/T 7714	Tan, Mingqian,Li, Xintong,Wu, Hao,et al. N-doped carbon dots derived from bovine serum albumin and formic acid with one- and two-photon fluorescence for live cell nuclear imaging[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2015,136:141-149.
APA	Tan, Mingqian,Li, Xintong,Wu, Hao,Wang, Beibei,&Wu, Jing.(2015).N-doped carbon dots derived from bovine serum albumin and formic acid with one- and two-photon fluorescence for live cell nuclear imaging.COLLOIDS AND SURFACES B-BIOINTERFACES,136,141-149.
MLA	Tan, Mingqian,et al."N-doped carbon dots derived from bovine serum albumin and formic acid with one- and two-photon fluorescence for live cell nuclear imaging".COLLOIDS AND SURFACES B-BIOINTERFACES 136(2015):141-149.