Screening and identification of DPP-IV inhibitory peptides from deer skin hydrolysates by an integrated approach of LC-MS/MS and in silico analysis

doi:10.1016/j.jff.2015.07.015

	Screening and identification of DPP-IV inhibitory peptides from deer skin hydrolysates by an integrated approach of LC-MS/MS and in silico analysis
	Jin, Yan 1; Yan, Jiaze 1; Yu, Yang 1,2; Qi, Yanxia 2
刊名	JOURNAL OF FUNCTIONAL FOODS
	2015-10-01
卷号	18 页码:344-357
关键词	Dipeptidyl Peptidase Iv Inhibitors Bioactive Peptides Liquid Chromatography And tAndem Mass Spectrometry (Lc-ms/ms) Deer Skin Collagen Integrated Approach
ISSN号	1756-4646
DOI	10.1016/j.jff.2015.07.015
文献子类	Article
英文摘要	An integrated approach combining liquid chromatography/tandem mass spectrometry (LC-MS/MS) with in silico analysis was used to screen dipeptidyl peptidase IV (DPP-IV) inhibitory peptides from deer skin hydrolysates. A total of 203, 244 and 60 peptides were identified from deer skin hydrolysates prepared with pepsin, pepsin + trypsin and pepsin + Alcalase, respectively, by LC-MS/MS. The percentages of peptides in the above mentioned hydrolysates containing a Pro residue at the penultimate position were 5.9, 20.9 and 20.0%, respectively. Five peptides with a Pro residue at the penultimate position were synthesized and assessed, and these five synthetic peptides possessed DPP-IV inhibitory activity with IC50 values from 83.3 to 1638.3 mu M. One of the evaluated peptides contained an oxidized methionine. The effects of peptide modification and length on the DPP-IV inhibitory activity of the peptides were assessed. The results suggest that the integrated approach was efficient in identifying novel bioactive peptides from hydrolysates. (C) 2015 Elsevier Ltd. All rights reserved.
WOS关键词	PROTEIN-DERIVED PEPTIDES ; INDUCED DIABETIC-RATS ; GELATIN HYDROLYSATE ; COLLAGEN HYDROLYSATE ; BIOACTIVE PEPTIDES ; GLYCEMIC CONTROL ; MASS-SPECTROMETRY ; DIETARY PROTEINS ; ENZYME ; ANTIOXIDANT
WOS研究方向	Food Science & Technology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000367757400032
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/171452]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Jin, Yan
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Dalian Ocean Univ, Dalian 116623, Peoples R China
推荐引用方式 GB/T 7714	Jin, Yan,Yan, Jiaze,Yu, Yang,et al. Screening and identification of DPP-IV inhibitory peptides from deer skin hydrolysates by an integrated approach of LC-MS/MS and in silico analysis[J]. JOURNAL OF FUNCTIONAL FOODS,2015,18:344-357.
APA	Jin, Yan,Yan, Jiaze,Yu, Yang,&Qi, Yanxia.(2015).Screening and identification of DPP-IV inhibitory peptides from deer skin hydrolysates by an integrated approach of LC-MS/MS and in silico analysis.JOURNAL OF FUNCTIONAL FOODS,18,344-357.
MLA	Jin, Yan,et al."Screening and identification of DPP-IV inhibitory peptides from deer skin hydrolysates by an integrated approach of LC-MS/MS and in silico analysis".JOURNAL OF FUNCTIONAL FOODS 18(2015):344-357.