Identification of Characteristic Fatty Acids to Quantify Triacylglycerols in Microalgae

doi:10.3389/fpls.2016.00162

	Identification of Characteristic Fatty Acids to Quantify Triacylglycerols in Microalgae
	Shen, Pei-Li 1,4; Wang, Hai-Tao 1,4; Pan, Yan-Fei 3; Meng, Ying-Ying 2; Wu, Pei-Chun 1; Xue, Song 1
刊名	FRONTIERS IN PLANT SCIENCE
	2016-02-22
卷号	7
关键词	Microalgae Triacylglycerols Neutral Lipids Characteristic Fattyacids Biofuels
ISSN号	1664-462X
DOI	10.3389/fpls.2016.00162
文献子类	Article
英文摘要	The fatty acid profiles of lipids from microalgae are unique. Polyunsaturated fatty acids are generally enriched in polar lipids, whereas saturated and monounsaturated fatty acids constitute the majority of fatty acids in triacylglycerols (TAG). Each species has characteristic fatty acids, and their content is positively or negatively correlated with TAGs. The marine oleaginous diatom Phaeodactylum tricornutum was used as the paradigm to determine the quantitative relationship between TAG and characteristic fatty acid content. Fatty acid profiles and TAG content of Phaeodactylum tricornutum were determined in a time course. C16:0/C16:1 and eicosapentaenoic acid (EPA, C20:5n3) were identified as characteristic fatty acids in TAGs and polar lipids, respectively. The percentage of those characteristic fatty acids in total fatty acids had a significant linear relationship with TAG content, and thus, the correlation coefficient presenting r(2) were 0.96, 0.94, and 0.97, respectively. The fatty acid-based method for TAG quantification could also be applied to other microalgae such as Nannochloropsis oceanica in which the r(2) of C16:0 and EPA were 0.94 and 0.97, respectively, and in Chlorella pyrenoidosa-values for C18:1 and C18:3 with TAG content were 0.91 and 0.99, repectively. This characteristic fatty acid-based method provided a distinct way to quantify TAGs in microalgae, by which TAGs could be measured precisely by immediate transesterification from wet biomass rather than using conventional methods. This procedure simplified the operation and required smaller samples than conventional methods.
WOS关键词	CHLAMYDOMONAS-REINHARDTII ; NANNOCHLOROPSIS-OCEANICA ; NITROGEN STARVATION ; ARACHIDONIC-ACID ; ACCUMULATION ; BIODIESEL ; LIPIDS ; DESATURASE
WOS研究方向	Plant Sciences
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000370543500001
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/171246]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Xue, Song
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Marine Bioengn Grp, Dalian, Peoples R China 2.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian, Peoples R China 3.Dalian Univ, Coll Environm & Chem Engn, Dept Environm Sci & Engn, Dalian 116012, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Shen, Pei-Li,Wang, Hai-Tao,Pan, Yan-Fei,et al. Identification of Characteristic Fatty Acids to Quantify Triacylglycerols in Microalgae[J]. FRONTIERS IN PLANT SCIENCE,2016,7.
APA	Shen, Pei-Li,Wang, Hai-Tao,Pan, Yan-Fei,Meng, Ying-Ying,Wu, Pei-Chun,&Xue, Song.(2016).Identification of Characteristic Fatty Acids to Quantify Triacylglycerols in Microalgae.FRONTIERS IN PLANT SCIENCE,7.
MLA	Shen, Pei-Li,et al."Identification of Characteristic Fatty Acids to Quantify Triacylglycerols in Microalgae".FRONTIERS IN PLANT SCIENCE 7(2016).