Novel Features of Eukaryotic Photosystem II Revealed by Its Crystal Structure Analysis from a Red Alga

doi:10.1074/jbc.M115.711689

	Novel Features of Eukaryotic Photosystem II Revealed by Its Crystal Structure Analysis from a Red Alga
	Ago, Hideo 3; Adachi, Hideyuki 4; Umena, Yasufumi 4,5,6; Tashiro, Takayoshi 7; Kawakami, Keisuke 5; Kamiya, Nobuo 5,7; Tian, Lirong 8; Han, Guangye 8; Kuang, Tingyun 8; Liu, Zheyi 1
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2016-03-11
卷号	291 期号:11 页码:5676-5687
关键词	Crystal Structure Crystallography Cyanobacteria Electron Transfer Complex Membrane Protein Photosynthesis Photosystem Ii X-ray Crystallography
ISSN号	0021-9258
DOI	10.1074/jbc.M115.711689
文献子类	Article
英文摘要	Photosystem II (PSII) catalyzes light-induced water splitting, leading to the evolution of molecular oxygen indispensible for life on the earth. The crystal structure of PSII from cyanobacteria has been solved at an atomic level, but the structure of eukaryotic PSII has not been analyzed. Because eukaryotic PSII possesses additional subunits not found in cyanobacterial PSII, it is important to solve the structure of eukaryotic PSII to elucidate their detailed functions, as well as evolutionary relationships. Here we report the structure of PSII from a red alga Cyanidium caldarium at 2.76 resolution, which revealed the structure and interaction sites of PsbQ, a unique, fourth extrinsic protein required for stabilizing the oxygen-evolving complex in the lumenal surface of PSII. The PsbQ subunit was found to be located underneath CP43 in the vicinity of PsbV, and its structure is characterized by a bundle of four up-down helices arranged in a similar way to those of cyanobacterial and higher plant PsbQ, although helices I and II of PsbQ were kinked relative to its higher plant counterpart because of its interactions with CP43. Furthermore, two novel transmembrane helices were found in the red algal PSII that are not present in cyanobacterial PSII; one of these helices may correspond to PsbW found only in eukaryotic PSII. The present results represent the first crystal structure of PSII from eukaryotic oxygenic organisms, which were discussed in comparison with the structure of cyanobacterial PSII.
WOS关键词	X-RAY CRYSTALLOGRAPHY ; OXYGEN-EVOLVING CENTER ; EXTRINSIC PROTEINS ; ANGSTROM RESOLUTION ; FUNCTIONAL-PROPERTIES ; CYANIDIUM-CALDARIUM ; CROSS-LINKING ; HIGHER-PLANTS ; PSBQ PROTEIN ; SYNECHOCOCCUS-VULCANUS
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
WOS记录号	WOS:000372551800019
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/171146]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Shen, Jian-Ren
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Aoyama Gakuin Univ, Dept Chem & Biol Sci, Sagamihara, Kanagawa 2525258, Japan 3.RIKEN SPring 8 Ctr, Mikazuki, Hyogo 6795148, Japan 4.Okayama Univ, Fac Sci, Grad Sch Nat Sci & Technol, Photosynthesis Res Ctr, Okayama 7008530, Japan 5.Osaka City Univ, Adv Res Inst Nat Sci & Technol OCARNA, Sumiyoshi Ku, 3-3-138 Sugimoto, Osaka 7008530, Japan 6.Japan Sci & Technol Agcy, Precursory Res Embryon Sci & Technol PRESTO, Tokyo 1020076, Japan 7.Osaka City Univ, Grad Sch Sci, Dept Chem, Sumiyoshi Ku, Osaka 5588585, Japan 8.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynthesis Res Ctr, Beijing 100093, Peoples R China 9.Tokyo Univ Sci, Fac Sci, Dept Biol, Shinjuku Ku, Tokyo 1628601, Japan
推荐引用方式 GB/T 7714	Ago, Hideo,Adachi, Hideyuki,Umena, Yasufumi,et al. Novel Features of Eukaryotic Photosystem II Revealed by Its Crystal Structure Analysis from a Red Alga[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2016,291(11):5676-5687.
APA	Ago, Hideo.,Adachi, Hideyuki.,Umena, Yasufumi.,Tashiro, Takayoshi.,Kawakami, Keisuke.,...&Shen, Jian-Ren.(2016).Novel Features of Eukaryotic Photosystem II Revealed by Its Crystal Structure Analysis from a Red Alga.JOURNAL OF BIOLOGICAL CHEMISTRY,291(11),5676-5687.
MLA	Ago, Hideo,et al."Novel Features of Eukaryotic Photosystem II Revealed by Its Crystal Structure Analysis from a Red Alga".JOURNAL OF BIOLOGICAL CHEMISTRY 291.11(2016):5676-5687.