Bioengineering three-dimensional culture model of human lung cancer cells: an improved tool for screening EGFR targeted inhibitors

doi:10.1039/c6ra00229c

	Bioengineering three-dimensional culture model of human lung cancer cells: an improved tool for screening EGFR targeted inhibitors
	Wang, Dan-Dan 2; Liu, Wei 1; Chang, Jing-Jie 1; Cheng, Xu 1; Zhang, Xiu-Zhen 1; Xu, Hong 1; Feng, Di 1; Yu, Li-Jun 1; Wang, Xiu-Li 1
刊名	RSC ADVANCES
	2016
卷号	6 期号:29 页码:24083-24090
ISSN号	2046-2069
DOI	10.1039/c6ra00229c
文献子类	Article
英文摘要	Cell models have been widely accepted to explore the mechanisms of tumorigenesis and predict drug response. Unfortunately, there is still a lack of accurate models that can highly recapitulate the important molecular events occurring in the human non-small-cell lung cancer patient's body. We hypothesize that the 3D microenvironment provided by collagen hydrogel would not only promote generation of tumor cultures that more closely resemble the in vivo tumor morphology, but also contribute to bioengineering a well-defined non-small-cell lung cancer culture model that potentially enhances the molecularly targeted drug evaluation. Here, we developed a more pathologically relevant, three-dimensional culture model of human lung cancer cells through embedding A549 cells within a collagen hydrogel. Our results showed that the collagen-embedded A549 cells formed numbers of cell aggregates that displayed good viability and more tissue-like morphology. A significantly increased expression level of EGF/EGFR was also observed in the 3D cultures. More importantly, the 3D culture had the ability to respond to treatment by the targeted inhibitor, as evidenced by its enhanced drug resistance and inhibited growth. Thus, this in vitro culture system effectively represents an improved culture model of human lung cancer and would potentially provide a robust tool for mechanism-based anti-lung cancer drug discovery and development.
WOS关键词	IN-VITRO ; TUMOR MICROENVIRONMENT ; GROWTH ; 3D ; PHENOTYPE ; SCAFFOLDS ; HALLMARKS ; HYDROGEL
WOS研究方向	Chemistry
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000372256800010
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/171136]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Yu, Li-Jun; Wang, Xiu-Li
作者单位	1.Dalian Med Univ, Coll Basic Med Sci, Dalian 116044, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Wang, Dan-Dan,Liu, Wei,Chang, Jing-Jie,et al. Bioengineering three-dimensional culture model of human lung cancer cells: an improved tool for screening EGFR targeted inhibitors[J]. RSC ADVANCES,2016,6(29):24083-24090.
APA	Wang, Dan-Dan.,Liu, Wei.,Chang, Jing-Jie.,Cheng, Xu.,Zhang, Xiu-Zhen.,...&Wang, Xiu-Li.(2016).Bioengineering three-dimensional culture model of human lung cancer cells: an improved tool for screening EGFR targeted inhibitors.RSC ADVANCES,6(29),24083-24090.
MLA	Wang, Dan-Dan,et al."Bioengineering three-dimensional culture model of human lung cancer cells: an improved tool for screening EGFR targeted inhibitors".RSC ADVANCES 6.29(2016):24083-24090.