Separation analysis of macrolide antibiotics with good performance on a positively charged C18HCE column

doi:10.1002/jssc.201500923

	Separation analysis of macrolide antibiotics with good performance on a positively charged C18HCE column
	Wei, Jie 1; Shen, Aijin 2; Yan, Jingyu 2; Jin, Gaowa 2; Yang, Bingcheng 1; Guo, Zhimou 2; Zhang, Feifang 1; Liang, Xinmiao 1,2
刊名	JOURNAL OF SEPARATION SCIENCE
	2016-03-01
卷号	39 期号:6 页码:1073-1081
关键词	Macrolides Peak Shape Positively Charged Surface Reversed-phase Liquid Chromatography
ISSN号	1615-9306
DOI	10.1002/jssc.201500923
文献子类	Article
英文摘要	The separation of basic macrolide antibiotics suffers from peak tailing and poor efficiency on traditional silica-based reversed-phase liquid chromatography columns. In this work, a C18HCE column with positively charged surface was applied to the separation of macrolides. Compared with an Acquity BEH C18 column, the C18HCE column exhibited superior performance in the aspect of peak shape and separation efficiency. The screening of mobile phase additives including formic acid, acetic acid and ammonium formate indicated that formic acid was preferable for providing symmetrical peak shapes. Moreover, the influence of formic acid content was investigated. Analysis speed and mass spectrometry compatibility were also taken into account when optimizing the separation conditions for liquid chromatography coupled with tandem mass spectrometry. The developed method was successfully utilized for the determination of macrolide residues in a honey sample. Azithromycin was chosen as the internal standard for the quantitation of spiramycin and tilmicosin, while roxithromycin was used for erythromycin, tylosin, clarithromycin, josamycin and acetylisovaleryltylosin. Good correlation coefficients (r(2) > 0.9938) for all macrolides were obtained. The intra-day and inter-day recoveries were 73.7-134.7% and 80.7-119.7% with relative standard deviations of 2.5-8.0% and 3.9-16.1%, respectively. Outstanding sensitivity with limits of quantitation (S/N 10) of 0.02-1 g/kg and limits of detection (S/N 3) of 0.01-0.5 g/kg were achieved.
WOS关键词	TANDEM MASS-SPECTROMETRY ; PHASE LIQUID-CHROMATOGRAPHY ; REVERSED-PHASE ; STATIONARY PHASES ; EMBEDDED GROUP ; DRUG RESIDUES ; RAW-MILK ; LC ; EXTRACTION ; RETENTION
WOS研究方向	Chemistry
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000373617100007
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/170712]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Guo, Zhimou; Zhang, Feifang
作者单位	1.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Wei, Jie,Shen, Aijin,Yan, Jingyu,et al. Separation analysis of macrolide antibiotics with good performance on a positively charged C18HCE column[J]. JOURNAL OF SEPARATION SCIENCE,2016,39(6):1073-1081.
APA	Wei, Jie.,Shen, Aijin.,Yan, Jingyu.,Jin, Gaowa.,Yang, Bingcheng.,...&Liang, Xinmiao.(2016).Separation analysis of macrolide antibiotics with good performance on a positively charged C18HCE column.JOURNAL OF SEPARATION SCIENCE,39(6),1073-1081.
MLA	Wei, Jie,et al."Separation analysis of macrolide antibiotics with good performance on a positively charged C18HCE column".JOURNAL OF SEPARATION SCIENCE 39.6(2016):1073-1081.