Application of a microfluidic-based perivascular tumor model for testing drug sensitivity in head and neck cancers and toxicity in endothelium

doi:10.1039/c6ra01456a

	Application of a microfluidic-based perivascular tumor model for testing drug sensitivity in head and neck cancers and toxicity in endothelium
	Jin, Dong 1; Ma, Xiaochi 2; Luo, Yong 3; Fang, Shimeng 1; Xie, Zhaorong 1; Li, Xiaojie 1; Qi, Dongyuan 4; Zhang, Fuyin 5; Kong, Jing 1; Li, Jiao 1
刊名	RSC ADVANCES
	2016
卷号	6 期号:35 页码:29598-29607
ISSN号	2046-2069
DOI	10.1039/c6ra01456a
文献子类	Article
英文摘要	A drug sensitivity test prior to clinical treatment is necessary for individualized cancer therapy. A useful in vitro model that mimics the in vivo tumor microenvironment is required to select optimal anti-cancer agents. To promote the microfluidic technology moving from the laboratory to the clinic, we developed a microfluidic-based perivascular tumor model that makes it possible to assess drug sensitivity in 3D cultured tumor spheroids and toxicity in endothelium in parallel. The sensitivity and toxicity of PTX, 5-FU, and CDDP were assessed using the microfluidic model. It was found that high concentrations of single drugs destroyed the human umbilical vein endothelial cell line (HUVEC) layer, although they can effectively induce apoptosis of head and neck cancer cells. A combination of low concentrations of these drugs presented a good curative effect on tumor cells and low toxicity to the HUVEC layer. Then, we applied the model to test anti-cancer drug sensitivity in cancer patients. Individual differences to candidate drug combinations were found among these patients. This suggests that different chemotherapy plans should be made for individual patients. This study demonstrated that the microfluidic model might be a useful platform for individual drug tests prior to clinical treatment.
WOS关键词	3D CELL-CULTURE ; TISSUE ; SPHEROIDS ; DISCOVERY ; CHIPS ; OPPORTUNITIES ; CHALLENGES ; COCULTURE ; ONCOLOGY ; DELIVERY
WOS研究方向	Chemistry
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000373029500069
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/170698]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Lin, Bingcheng; Liu, Tingjiao
作者单位	1.Dalian Med Univ, Coll Stomatol, West Sect 9,South Rd Lvshun, Dalian 116044, Peoples R China 2.Dalian Med Univ, Coll Pharm, West Sect 9,South Rd Lvshun, Dalian 116044, Peoples R China 3.Dalian Univ Technol, Fac Chem Environm & Biol Sci & Technol, 2 Linggong Rd, Dalian 116024, Peoples R China 4.Dalian Med Univ, Affiliated Hosp 1, 222 Zhongshan Rd, Dalian 116011, Peoples R China 5.Dalian Med Univ, Affiliated Hosp 2, 465 Zhongshan Rd, Dalian 116011, Peoples R China 6.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, 457 Zhongshan Rd, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Jin, Dong,Ma, Xiaochi,Luo, Yong,et al. Application of a microfluidic-based perivascular tumor model for testing drug sensitivity in head and neck cancers and toxicity in endothelium[J]. RSC ADVANCES,2016,6(35):29598-29607.
APA	Jin, Dong.,Ma, Xiaochi.,Luo, Yong.,Fang, Shimeng.,Xie, Zhaorong.,...&Liu, Tingjiao.(2016).Application of a microfluidic-based perivascular tumor model for testing drug sensitivity in head and neck cancers and toxicity in endothelium.RSC ADVANCES,6(35),29598-29607.
MLA	Jin, Dong,et al."Application of a microfluidic-based perivascular tumor model for testing drug sensitivity in head and neck cancers and toxicity in endothelium".RSC ADVANCES 6.35(2016):29598-29607.