Synthesis of Zwitterionic Polymer Particles via Combined Distillation Precipitation Polymerization and Click Chemistry for Highly Efficient Enrichment of Glycopeptide

doi:10.1021/acsami.6b06343

	Synthesis of Zwitterionic Polymer Particles via Combined Distillation Precipitation Polymerization and Click Chemistry for Highly Efficient Enrichment of Glycopeptide
	Liu, Jianxi 1,2; Yang, Kaiguang 1; Shao, Wenya 1; Li, Senwu 1; Wu, Qi 1; Zhang, Shen 1; Qu, Yanyan 1; Zhang, Lihua 1; Zhang, Yukui 1
刊名	ACS APPLIED MATERIALS & INTERFACES
	2016-08-31
卷号	8 期号:34 页码:22018-22024
关键词	Core-shell Structures Separation Click Chemistry Nanoparticles Glycopeptides
ISSN号	1944-8244
DOI	10.1021/acsami.6b06343
文献子类	Article
英文摘要	Because, of the low abundance of glycopeptide in natural biological samples, methods for efficient and selective enrichment of glycopeptides play a significant role in mass spectrometry (MS)-based glycoproteomics. In this study, a novel kind of zwitterionic hydrophilic interaction chromatography polymer particles, namely, poly(N,N-methylenebisacrylamide-co-methacrylic acid)@l-Cys (poly(MBAAm-co-MAA)@L-Cys), for the enrichment of glycopeptides was synthesized by a facile and efficient approach that combined distillation precipitation polymerization (DPP) and "thiol-ene" click reaction. In the DPP approach, residual vinyl groups explored outside the core with high density, then the functional ligand cysteine was immobilized onto the surface of core particles by highly efficient thiol-ene click reaction. Taking advantage of the unique structure of poly(MBAAm-co-MAA)@L-Cys, the resulting particles possess remarkable enrichment selectivity for glycopeptides from the tryptic digested human immunoglobulin G. The polymer particles were successfully employed for the analysis of human plasma, and 208 unique glycopeptides corresponding to 121 glycoproteins were reliably identified in triple independent nano-LC-MS/MS runs. The selectivity toward glycopeptides of these particles poly(MBAAm-co-MAA)@L-Cys is similar to 2 times than that of the commercial beads. These results demonstrated that these particles had great potential for large-scale glycoproteomics research. Moreover, the strategy with the combination of DPP and thiol-ene click chemistry might be a facile method to produce functional polymer particles for bioenrichment application.
WOS关键词	FUNCTIONALIZED MAGNETIC NANOPARTICLES ; SELECTIVE ENRICHMENT ; MASS-SPECTROMETRY ; STATIONARY-PHASE ; CHROMATOGRAPHY ; GLYCOSYLATION ; GLYCAN ; DEGLYCOSYLATION ; GLYCOPROTEINS ; ADSORBENTS
WOS研究方向	Science & Technology - Other Topics ; Materials Science
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000382514100022
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/170092]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Zhang, Lihua
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&D Ctr, Dalian 116023, Peoples R China 2.Fujian Normal Univ, Coll Environm Sci & Engn, Fuzhou 350007, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jianxi,Yang, Kaiguang,Shao, Wenya,et al. Synthesis of Zwitterionic Polymer Particles via Combined Distillation Precipitation Polymerization and Click Chemistry for Highly Efficient Enrichment of Glycopeptide[J]. ACS APPLIED MATERIALS & INTERFACES,2016,8(34):22018-22024.
APA	Liu, Jianxi.,Yang, Kaiguang.,Shao, Wenya.,Li, Senwu.,Wu, Qi.,...&Zhang, Yukui.(2016).Synthesis of Zwitterionic Polymer Particles via Combined Distillation Precipitation Polymerization and Click Chemistry for Highly Efficient Enrichment of Glycopeptide.ACS APPLIED MATERIALS & INTERFACES,8(34),22018-22024.
MLA	Liu, Jianxi,et al."Synthesis of Zwitterionic Polymer Particles via Combined Distillation Precipitation Polymerization and Click Chemistry for Highly Efficient Enrichment of Glycopeptide".ACS APPLIED MATERIALS & INTERFACES 8.34(2016):22018-22024.