Insight of In Vitro Small-Interfering RNA Release From Chitosan Nanoparticles Under Enzymolysis With Forster Resonance Energy Transfer Analysis

doi:10.1002/jps.24698

	Insight of In Vitro Small-Interfering RNA Release From Chitosan Nanoparticles Under Enzymolysis With Forster Resonance Energy Transfer Analysis
	Ma, Xiaojun 1; Yu, Weiting 1; Zhang, Demeng 1,2; Song, Yizhe 1,2; Wang, Yu 1,2; Liu, Xiudong 3
刊名	JOURNAL OF PHARMACEUTICAL SCIENCES
	2016
卷号	105 期号:1 页码:301-307
关键词	Nonviral Gene Delivery Rnai Sirna Chitosan Nanoparticles Nanotechnology
ISSN号	0022-3549
DOI	10.1002/jps.24698
文献子类	Article
英文摘要	Small-interfering RNA (siRNA)-mediated gene silencing with the aid of chitosan (CS)-based carriers has shown efficient and reliable outcome in vitro, but the gene silencing efficiency in vivo is still limited. It is of great importance to balance the protection and release of siRNA from nanoparticles (NPs) so as to achieve high efficiency. However, siRNA release profile from CS/siRNA NPs has been rarely concerned. Here, Forster resonance energy transfer technique was adopted for in vitro investigation of siRNA release from CS NPs in lysozyme-contained buffer. The results clearly showed that siRNA molecules experienced a fast and short release phase under lysozyme competition to both CS and siRNA, and then a slow and long release under lysozyme degradation on CS. Moreover, lysozyme competition played more important role than enzymolysis on trigging siRNA release. This preliminary study of siRNA release is the first step to get insight of in vivo siRNA release mechanism from CS/siRNA NPs, which will be helpful to adjust the design of CS/siRNA NPs for balancing the protection and release of siRNA molecules. (C) 2016 American Pharmacists Association (R). Published by Elsevier Inc. All rights reserved.
WOS关键词	SIRNA DELIVERY ; INTRACELLULAR TRAFFICKING ; GENE DELIVERY ; LYSOZYME ; CHITIN ; THERAPEUTICS ; FORMULATION ; POLYPLEXES ; SYSTEMS ; PROBES
WOS研究方向	Pharmacology & Pharmacy ; Chemistry
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000381768000037
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/170020]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Yu, Weiting
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Dalian Univ, Coll Environm & Chem Engn, Dalian 116622, Peoples R China
推荐引用方式 GB/T 7714	Ma, Xiaojun,Yu, Weiting,Zhang, Demeng,et al. Insight of In Vitro Small-Interfering RNA Release From Chitosan Nanoparticles Under Enzymolysis With Forster Resonance Energy Transfer Analysis[J]. JOURNAL OF PHARMACEUTICAL SCIENCES,2016,105(1):301-307.
APA	Ma, Xiaojun,Yu, Weiting,Zhang, Demeng,Song, Yizhe,Wang, Yu,&Liu, Xiudong.(2016).Insight of In Vitro Small-Interfering RNA Release From Chitosan Nanoparticles Under Enzymolysis With Forster Resonance Energy Transfer Analysis.JOURNAL OF PHARMACEUTICAL SCIENCES,105(1),301-307.
MLA	Ma, Xiaojun,et al."Insight of In Vitro Small-Interfering RNA Release From Chitosan Nanoparticles Under Enzymolysis With Forster Resonance Energy Transfer Analysis".JOURNAL OF PHARMACEUTICAL SCIENCES 105.1(2016):301-307.