Nitrogen-doped graphene quantum dots-labeled epitope imprinted polymer with double templates via the metal chelation for specific recognition of cytochrome c

doi:10.1016/j.bios.2016.12.040

	Nitrogen-doped graphene quantum dots-labeled epitope imprinted polymer with double templates via the metal chelation for specific recognition of cytochrome c
	He, Xi-Wen 1; Zhang, Yu-Kui 1,3; Yan, Yun-Jing 1; Li, Wen-You 1,2
刊名	BIOSENSORS & BIOELECTRONICS
	2017-05-15
卷号	91 页码:253-261
关键词	Surface Imprinting Nitrogen-doped Graphene Quantum Dots Epitope Imprinting Metal Chelation Fluorescent Sensor
ISSN号	0956-5663
DOI	10.1016/j.bios.2016.12.040
文献子类	Article
英文摘要	A novel fluorescent sensor nitrogen-doped graphene quantum dots (N-GQDs)/SiO2/molecular imprinting polymer ( N-GQDs/SiO2/MIP) was fabricated by surface imprinting and epitope imprinting to recognize and detect the target protein cytochrome c (Cyt C) with fluorescence quenching. In the polymerization process, the C- and N-terminal nonapeptides of Cyt C were selected as the double templates which were fixed by functional monomer (zinc acrylate) through metal chelation and steady six-membered ring. The linear range of fluorescence quenching for this receptor towards Cyt C was 0.20-60 mu M, and the detection limit was 0.11 mu M. The precision for six times replicate determination of Cyt C at 30 M was 1.20%, and the imprinting factor (IF) was 3.06. The recoveries of the material to Cyt C in urine were 99.3-114.0%. In brief, this work proposed a strategy to prepare a new type fluorescent imprinting polymer based on N-GQDs and provided an attractive perspective for the detection of protein by using the combination of N-GQDs and molecular imprinting technique.
WOS关键词	ONE-POT SYNTHESIS ; AQUEOUS-MEDIA ; FLUORESCENCE DETECTION ; PHENYLPHOSPHONIC ACID ; PROTEIN RECOGNITION ; SERUM-ALBUMIN ; BOVINE SERUM ; NANOPARTICLES ; SURFACE ; PEPTIDES
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种	英语
出版者	ELSEVIER ADVANCED TECHNOLOGY
WOS记录号	WOS:000395599000038
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/169326]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Zhang, Yu-Kui; Li, Wen-You
作者单位	1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin Key Lab Biosensing & Mol Recognit, Coll Chem,Res Ctr Analyt Sci, Tianjin 300071, Peoples R China 2.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Natl Chromatog Res & Anal Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	He, Xi-Wen,Zhang, Yu-Kui,Yan, Yun-Jing,et al. Nitrogen-doped graphene quantum dots-labeled epitope imprinted polymer with double templates via the metal chelation for specific recognition of cytochrome c[J]. BIOSENSORS & BIOELECTRONICS,2017,91:253-261.
APA	He, Xi-Wen,Zhang, Yu-Kui,Yan, Yun-Jing,&Li, Wen-You.(2017).Nitrogen-doped graphene quantum dots-labeled epitope imprinted polymer with double templates via the metal chelation for specific recognition of cytochrome c.BIOSENSORS & BIOELECTRONICS,91,253-261.
MLA	He, Xi-Wen,et al."Nitrogen-doped graphene quantum dots-labeled epitope imprinted polymer with double templates via the metal chelation for specific recognition of cytochrome c".BIOSENSORS & BIOELECTRONICS 91(2017):253-261.