Effects of gelling bath on the physical properties of alginate gel beads and the biological characteristics of entrapped HepG2 cells

doi:10.1002/bab.1585

	Effects of gelling bath on the physical properties of alginate gel beads and the biological characteristics of entrapped HepG2 cells
	Wu, Huijian 2; Sun, Guangwei 3; Sun, Dongsheng 1,2,3; Liu, Yang 3; Wu, Hao 1,3; Ren, Ying 1,4; Ma, Xiaojun 4
刊名	BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
	2018-03-01
卷号	65 期号:2 页码:263-273
关键词	Alginate Gel Bead Gelling Bath Mass Transfer Proliferation Three-dimensional Culture
ISSN号	0885-4513
DOI	10.1002/bab.1585
文献子类	Article
英文摘要	Optimizing alginate gel beads is necessary to support the survival, proliferation, and function of entrapped hepatocytes. In this study, gelling bath was modified by decreasing calcium ion concentration and increasing sodium ion concentration. Alginate gel beads (using 36% G sodium alginate) prepared in the modified gelling bath had more homogeneous structure and better mass transfer properties compared with the traditional gelling bath that contains only calcium ions. Moreover, alginate gel beads generated in the modified gelling bath could significantly promote the HepG2 cell proliferation and the growth of cell spheroids, and maintain the albumin secretion ability similar to alginate gel beads prepared in the traditional gelling bath with only calcium ions. The mass transfer properties and cell proliferation were similar in ALG beads with different M/G ratio (36% G and 55% G) generated in the modified gelling bath, whereas they were significantly increased compared with alginate gel beads (55% G) in traditional gelling bath. These results indicated that adjusting the gelling bath was a simple and convenient method to enhance the mass transfer properties of alginate gel beads for 3D hepatocyte culture, which might provide more hepatocytes for the bioartificial liver support system.
WOS关键词	BIOARTIFICIAL LIVER ; IMMOBILIZATION MATERIAL ; SPHEROID CULTURE ; C3A CELLS ; MICROCAPSULES ; MATRIX ; MODEL ; HEPATOCYTES ; SUPPORT ; POLYCATION
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种	英语
出版者	WILEY
WOS记录号	WOS:000430115900019
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/169036]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Wu, Huijian; Sun, Guangwei; Liu, Yang
作者单位	1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Dalian Univ Technol, Sch Life Sci & Biotechnol, 2 Ling Gong Rd, Dalian 116024, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Sci Res Ctr Translat Med, 457 Zhongshan Rd, Dalian 116023, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Wu, Huijian,Sun, Guangwei,Sun, Dongsheng,et al. Effects of gelling bath on the physical properties of alginate gel beads and the biological characteristics of entrapped HepG2 cells[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2018,65(2):263-273.
APA	Wu, Huijian.,Sun, Guangwei.,Sun, Dongsheng.,Liu, Yang.,Wu, Hao.,...&Ma, Xiaojun.(2018).Effects of gelling bath on the physical properties of alginate gel beads and the biological characteristics of entrapped HepG2 cells.BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,65(2),263-273.
MLA	Wu, Huijian,et al."Effects of gelling bath on the physical properties of alginate gel beads and the biological characteristics of entrapped HepG2 cells".BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 65.2(2018):263-273.