Thermosensitive Metal Chelation Dual-Template Epitope Imprinting Polymer Using Distillation-Precipitation Polymerization for Simultaneous Recognition of Human Serum Albumin and Transferrin

doi:10.1021/acsami.8b00327

	Thermosensitive Metal Chelation Dual-Template Epitope Imprinting Polymer Using Distillation-Precipitation Polymerization for Simultaneous Recognition of Human Serum Albumin and Transferrin
	Qin, Ya-Ping 2; Jia, Chao 2; He, Xi-Wen 2; Li, Wen-You 2,3; Zhang, Yu-Kui 1,2
刊名	ACS APPLIED MATERIALS & INTERFACES
	2018-03-14
卷号	10 期号:10 页码:9060-9068
关键词	Dual-template Epitope Imprinting Molecular Imprinting Polymer Thermosensitive Recognition Metal Chelation Imprinting Distillation-precipitation Polymerization
ISSN号	1944-8244
DOI	10.1021/acsami.8b00327
文献子类	Article
英文摘要	A new type of thermosensitive dual-template epitope molecular imprinting polymer was prepared and coated on magnetic carbon nanotubes (MCNTs@D-EMIP) for simultaneous recognition of human serum albumin (HSA) and transferrin (Trf) via the strategies of dual-template epitope imprinting, metal chelation imprinting, and distillation precipitation polymerization (DPP). C-terminal peptides of HSA and C-terminal peptides of Trf were selected as templates, zinc acrylate and N-isopropylacrylamide were used as functional monomers, and MCNTs@D-EMIP was prepared by the method of DPP. The two types of template epitopes were immobilized by metal chelation and six-membered ring formed with zinc acylate. MCNTs@D-EMIP was prepared in only 30 min, which was much shorter than other polymerization methods. The resultant MCNTs@D-EMIP showed excellent specific recognition ability toward HSA and Trf. The adsorption amounts of MCNTs@D-EMIP for HSA and Trf were 103.67 and 68.48 mg(-1) and the imprinting factors were 2.57 and 2.17, respectively. In addition, MCNTs@D-EMIP displayed a thermosensitive property to realize temperature controlled recognition and release of target proteins. Furthermore, the results of high-performance liquid chromatography analysis proved that MCNTs@D-EMIP could be applied to specifically recognize two types of targets simultaneously in the biosample. The proposed strategy provided a preparation method for the thermosensitive dual-template epitope imprinting polymer via dual-template imprinting, metal chelation imprinting, and DPP.
WOS关键词	PROTEIN-PROTEIN INTERACTIONS ; DRUG-DELIVERY ; SURFACE ; NANOPARTICLES ; FLUOROQUINOLONES ; NANOCOMPOSITES ; MICROSPHERES ; PARTICLES ; PEPTIDES ; AFFINITY
WOS研究方向	Science & Technology - Other Topics ; Materials Science
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000427910800072
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/168920]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Li, Wen-You
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China 2.Nankai Univ, Tianjin Key Lab Biosensing & Mol Recognit, State Key Lab Med Chem Biol, Coll Chem,Res Ctr Analyt Sci, Tianjin 300071, Peoples R China 3.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China
推荐引用方式 GB/T 7714	Qin, Ya-Ping,Jia, Chao,He, Xi-Wen,et al. Thermosensitive Metal Chelation Dual-Template Epitope Imprinting Polymer Using Distillation-Precipitation Polymerization for Simultaneous Recognition of Human Serum Albumin and Transferrin[J]. ACS APPLIED MATERIALS & INTERFACES,2018,10(10):9060-9068.
APA	Qin, Ya-Ping,Jia, Chao,He, Xi-Wen,Li, Wen-You,&Zhang, Yu-Kui.(2018).Thermosensitive Metal Chelation Dual-Template Epitope Imprinting Polymer Using Distillation-Precipitation Polymerization for Simultaneous Recognition of Human Serum Albumin and Transferrin.ACS APPLIED MATERIALS & INTERFACES,10(10),9060-9068.
MLA	Qin, Ya-Ping,et al."Thermosensitive Metal Chelation Dual-Template Epitope Imprinting Polymer Using Distillation-Precipitation Polymerization for Simultaneous Recognition of Human Serum Albumin and Transferrin".ACS APPLIED MATERIALS & INTERFACES 10.10(2018):9060-9068.