Adenosine Phosphate Functionalized Magnetic Mesoporous Graphene Oxide Nanocomposite for Highly Selective Enrichment of Phosphopeptides

doi:10.1021/acssuschemeng.7b03607

	Adenosine Phosphate Functionalized Magnetic Mesoporous Graphene Oxide Nanocomposite for Highly Selective Enrichment of Phosphopeptides
	Su, Jie 1; He, Xiwen 1; Chen, Langxing 1,2; Zhang, Yukui 1,3
刊名	ACS SUSTAINABLE CHEMISTRY & ENGINEERING
	2018-02-01
卷号	6 期号:2 页码:2188-2196
关键词	Magnetic Graphene Oxide Mesoporous Silica Adenosine Triphosphate Titanium Ion Phosphopeptide Enrichment
ISSN号	2168-0485
DOI	10.1021/acssuschemeng.7b03607
文献子类	Article
英文摘要	Developing an efficient strategy to enrich the low abundance phosphopeptides before mass spectrometry detection is a vital preprocessing step in phosphoproteomics. In this work, we synthesized an adenosine phosphate-Ti4+ functionalized magnetic mesoporous graphene oxide nanocomposite (denoted as MG@ mSiO(2)-ATP-Ti4+) to selectively extract phosphorylated peptides from complex biological samples based on the immobilized metal ion affinity chromatography (IMAC). Mesoporous silica was coated on the substrate material of magnetic graphene oxide and then the ATP containing three phosphate groups was grafted on the inwall of mesoporous channels as chelating ligands to immobilize the Ti4+ cations. With favorable properties, such as large surface area and good hydrophilicity and size-exclusion effect, the MG@mSiO(2)-ATP-Ti4+ exhibited excellent sensitivity and selectivity toward phosphopeptides whether in low concentration of beta-casein digest (20 amol mu L-1, 4 fmol) or the digest mixture of beta-casein and bovine serum albumin (with molar ratio of 1:1000) as well as good reusability. Furthermore, MG@mSiO(2)-ATP-Ti4+ could also be applied in the selective enrichment of phosphorylated peptides from nonfat milk digest and human saliva and serum.
WOS关键词	GRAPHENE/MESOPOROUS SILICA COMPOSITES ; AFFINITY-CHROMATOGRAPHY PLATFORM ; MASS-SPECTROMETRY ANALYSIS ; N-LINKED GLYCAN ; ENDOGENOUS PHOSPHOPEPTIDES ; DESIGNED SYNTHESIS ; PHOSPHOPROTEOME ANALYSIS ; FACILE PREPARATION ; ORGANIC FRAMEWORK ; HYBRID MATERIAL
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Engineering
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000424728300073
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/168702]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Chen, Langxing
作者单位	1.Nankai Univ, Coll Chem, Tianjin Key Lab Biosensing & Mol Recognit, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China 2.Nankai Univ, Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Su, Jie,He, Xiwen,Chen, Langxing,et al. Adenosine Phosphate Functionalized Magnetic Mesoporous Graphene Oxide Nanocomposite for Highly Selective Enrichment of Phosphopeptides[J]. ACS SUSTAINABLE CHEMISTRY & ENGINEERING,2018,6(2):2188-2196.
APA	Su, Jie,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2018).Adenosine Phosphate Functionalized Magnetic Mesoporous Graphene Oxide Nanocomposite for Highly Selective Enrichment of Phosphopeptides.ACS SUSTAINABLE CHEMISTRY & ENGINEERING,6(2),2188-2196.
MLA	Su, Jie,et al."Adenosine Phosphate Functionalized Magnetic Mesoporous Graphene Oxide Nanocomposite for Highly Selective Enrichment of Phosphopeptides".ACS SUSTAINABLE CHEMISTRY & ENGINEERING 6.2(2018):2188-2196.