Probing impaired neurogenesis in human brain organoids exposed to alcohol

doi:10.1039/c7ib00105c

	Probing impaired neurogenesis in human brain organoids exposed to alcohol
	Zhu, Yujuan 2,3,4; Wang, Li 2,4; Yin, Fangchao 2,3,4; Yu, Yue 2,3,4; Wang, Yaqing 2,3,4; Shepard, Matthew J.1,5; Zhuang, Zhengping 5; Qin, Jianhua 2,3,6
刊名	INTEGRATIVE BIOLOGY
	2017-12-01
卷号	9 期号:12 页码:968-978
ISSN号	1757-9694
DOI	10.1039/c7ib00105c
文献子类	Article
英文摘要	The fetal brain is highly vulnerable to ethanol exposure, which can trigger various long-term neuronal disabilities and cognitive dysfunctions. However, a comprehensive understanding of fetal brain development under ethanol exposure is challenging due to the limitations of animal models. Here, we propose a human induced pluripotent stem cell (hiPSC)-based 3D brain organoid model, and explore the mechanisms underlying neural dysfunctions in prenatal alcohol exposure (PAE) in vitro. Brain organoids were examined to resemble brain organogenesis in vivo at early stages during gestation, with specific features of neuronal differentiation, brain regionalization, and cortical organization. With ethanol exposure, the brain organoids displayed attenuated neurite outgrowth and skewed neural maturation. Transcriptome analysis identified a series of new markedly altered genes and enriched pathways, such as GSX2, RSPO2, and the Hippo signaling pathway. These genes or pathways, to our knowledge, were reported to be involved in ethanol-induced impaired neurogenesis for the first time. Our new findings might facilitate better understanding of the various postnatal neural disorders observed in individuals with PAE.
WOS关键词	PLURIPOTENT STEM-CELLS ; HUMAN NEOCORTEX ; IN-VITRO ; ETHANOL ; DISEASE ; CORTEX ; EXPRESSION ; REGULATORS ; RECEPTORS ; NEURONS
WOS研究方向	Cell Biology
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000417759500007
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/168436]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Qin, Jianhua
作者单位	1.Univ Virginia, Dept Neurol Surg, Charlottesville, VA USA 2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Beijing, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Beijing, Peoples R China 5.NINDS, Surg Neurol Branch, NIH, Bldg 36,Rm 4D04, Bethesda, MD 20892 USA 6.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Yujuan,Wang, Li,Yin, Fangchao,et al. Probing impaired neurogenesis in human brain organoids exposed to alcohol[J]. INTEGRATIVE BIOLOGY,2017,9(12):968-978.
APA	Zhu, Yujuan.,Wang, Li.,Yin, Fangchao.,Yu, Yue.,Wang, Yaqing.,...&Qin, Jianhua.(2017).Probing impaired neurogenesis in human brain organoids exposed to alcohol.INTEGRATIVE BIOLOGY,9(12),968-978.
MLA	Zhu, Yujuan,et al."Probing impaired neurogenesis in human brain organoids exposed to alcohol".INTEGRATIVE BIOLOGY 9.12(2017):968-978.