Plasma bile acid changes in type 2 diabetes correlated with insulin secretion in two-step hyperglycemic clamp

doi:10.1111/1753-0407.12771

	Plasma bile acid changes in type 2 diabetes correlated with insulin secretion in two-step hyperglycemic clamp
	Xie, Xiaoyan 2; Ma, Jing 1; Xu, Min 2; Zhao, Xinjie 3; Gu, Weiqiong 2; Hong, Jie 2; Xu, Guowang 3; Wang, Weiqing 2; Ning, Guang 2; Gu, Yanyun 2
刊名	JOURNAL OF DIABETES
	2018-11-01
卷号	10 期号:11 页码:874-885
关键词	bile acid hyperglycemic clamp insulin secretion type 2 diabetes mellitus
ISSN号	1753-0393
DOI	10.1111/1753-0407.12771
通讯作者	Gu, Yanyun(gyy11251@rjh.com.cn) ; Zhang, Yifei(feifei-a@163.com)
英文摘要	Background: Bile acids (BAs) conduct crucial signals in human metabolism. Correlations between changes in plasma BA concentrations, insulin secretion defects, and progression of type 2 diabetes mellitus (T2DM) in humans have not been sufficiently investigated. This study explored the trajectories of changes in human plasma BA concentrations and their association with insulin secretion dynamics during a two-step hyperglycemic clamp. Methods: Eleven healthy subjects with normal glucose tolerance (NGT) and 33 drug-naive T2DM subjects were enrolled in the study. The two-step hyperglycemic clamp consisted of a classic clamp as Step 1 with fasting, followed by a Step 2 clamp after ingestion of a carbohydrate meal, illustrating basal and incretin-amplified insulin responses to glucose. Plasma BA were assayed using liquid chromatography-tandem mass spectrometry. Nine T2DM subjects were followed-up, and the two-step clamp was repeated after 3 months sulfonylurea treatment. Results: Ursodeoxycholic acid (UDCA) was lower and lithocholic acid (LCA) and taurocholic acid (TCA) were higher in T2DM compared with NGT subjects. The dynamics of plasma UDCA concentrations and the UDCA/LCA ratio was positively correlated with insulin secretion in T2DM subjects and were corrected after treatment. Moreover, fasting ratios of UDCA/LCA and unconjugated/conjugated BAs were correlated with the first phase of insulin secretion in T2DM subjects. Conclusion: The abnormal BA composition in T2DM subjects and its correlation with insulin secretion during the clamp suggest an interaction between BA signals and insulin secretion capacity, and the potential to use fasting plasma BA composition indices to predict and evaluate the progression and prognosis of T2DM.
资助项目	National Key R&D Program of China[2016YFC0901200] ; National Basic Research Program of China[2015CB5536003] ; National Nature Science Foundation of China[81621061] ; National Nature Science Foundation of China[81670761]
WOS关键词	BETA-CELL FUNCTION ; URSODEOXYCHOLIC ACID ; GLUCOSE-TOLERANCE ; METABOLISM ; SENSITIVITY ; OBESITY ; ACTIVATION ; RESISTANCE ; MICROBIOTA ; PHENOTYPE
WOS研究方向	Endocrinology & Metabolism
语种	英语
出版者	WILEY
WOS记录号	WOS:000446325600008
资助机构	National Key R&D Program of China ; National Key R&D Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/166938]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Gu, Yanyun; Zhang, Yifei
作者单位	1.Shanghai Jiao Tong Univ, Dept Endocrinol, Med Sch, Renji Hosp, 10th Bldg,197 RuiJin Er Rd, Shanghai 200025, Peoples R China 2.Shanghai Jiao Tong Univ, Shanghai Inst Endocrine & Metab Dis, Ruijin Hosp,State Key Lab Med Genom, Shanghai Natl Res Ctr Endocrine & Metab Dis,Sch M, Shanghai, Peoples R China 3.Chinese Acad Sci, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Xie, Xiaoyan,Ma, Jing,Xu, Min,et al. Plasma bile acid changes in type 2 diabetes correlated with insulin secretion in two-step hyperglycemic clamp[J]. JOURNAL OF DIABETES,2018,10(11):874-885.
APA	Xie, Xiaoyan.,Ma, Jing.,Xu, Min.,Zhao, Xinjie.,Gu, Weiqiong.,...&Deng, Yuying.(2018).Plasma bile acid changes in type 2 diabetes correlated with insulin secretion in two-step hyperglycemic clamp.JOURNAL OF DIABETES,10(11),874-885.
MLA	Xie, Xiaoyan,et al."Plasma bile acid changes in type 2 diabetes correlated with insulin secretion in two-step hyperglycemic clamp".JOURNAL OF DIABETES 10.11(2018):874-885.