An Efficient Approach for Selective Enrichment of Histone Modification Readers Using Self-Assembled Multivalent Photoaffinity Peptide Probes

doi:10.1021/acs.analchem.8b02342

	An Efficient Approach for Selective Enrichment of Histone Modification Readers Using Self-Assembled Multivalent Photoaffinity Peptide Probes
	Zhang, Kai 3; Zhai, Guijin 3; Dong, Hanyang 3; Guo, Zhenchang 3; Feng, Wei 4; Jin, Jin 1; Zhang, Tao 4; Chen, Cong 3; Chen, Pu 3; Tian, Shanshan 3
刊名	ANALYTICAL CHEMISTRY
	2018-10-02
卷号	90 期号:19 页码:11385-11392
ISSN号	0003-2700
DOI	10.1021/acs.analchem.8b02342
通讯作者	Zhang, Kai(kzhang@tmu.edu.cn)
英文摘要	Histone post-translational modifications (HPTMs) provide signaling platforms to recruit proteins or protein complexes (e.g., transcription factors, the so-called "readers" of the histone code), changing DNA accessibility in the regulation of gene expression. Thus, it is an essential task to identify HPTM readers for understanding of epigenetic regulation. Herein we designed and prepared a novel HPTM probe based on self-assembled multivalent photo-cross-linking technique for selective enrichment and identification of HPTM readers. By use of trimethylation of histone H3 lysine 4, we showcased that the functionalized HPTM probe was able to capture its reader with high enrichment efficiency and remarkable specificity even in a complex environment. Notably, this approach was readily applicable for exploring crosstalk among multiple HPTMs. Combining the probes with a mass spectrometry-based proteomic approach, our approach reached a fairly high coverage of known H3K4me3 readers. We further demonstrated that the HPTM probes can enrich a new type of HPTM readers and uncovered several novel putative binders of crotonylation of histone H3 lysine 9, expanding the repertoire of readers for this epigenetic mark. More broadly, our work provides a general strategy for rapid and robust interrogating HPTM readers and will be of great importance to elucidate epigenetic mechanism in regulating gene activity.
资助项目	National Natural Science Foundation of China[21874100] ; National Natural Science Foundation of China[21275077] ; National Natural Science Foundation of China[21705067] ; National Basic Research Program of China[2016YFC0903000] ; Talent Excellence Program from Tianjin Medical University
WOS关键词	MODIFIED GOLD NANOPARTICLES ; PHD FINGER ; YEATS DOMAIN ; PROTEINS ; CROTONYLATION ; TRANSCRIPTION ; IDENTIFICATION ; ACETYLATION ; PROTEOMICS ; DNA
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000446542600032
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; Talent Excellence Program from Tianjin Medical University ; Talent Excellence Program from Tianjin Medical University ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; Talent Excellence Program from Tianjin Medical University ; Talent Excellence Program from Tianjin Medical University ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; Talent Excellence Program from Tianjin Medical University ; Talent Excellence Program from Tianjin Medical University ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; Talent Excellence Program from Tianjin Medical University ; Talent Excellence Program from Tianjin Medical University
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/166812]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Zhang, Kai
作者单位	1.Nankai Univ, Coll Pharm, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian 116023, Peoples R China 3.Tianjin Med Univ, Canc Inst & Hosp,Tianjin Key Lab Med Epigenet,Col, Key Lab Breast Canc Prevent & Treatment,Minist Ed, Dept Biochem & Mol Biol,Key Lab Immune Microenvir, Tianjin 300070, Peoples R China 4.Tianjin Med Univ, Sch Biomed Engn, Tianjin 300070, Peoples R China 5.Univ Freiburg, Inst Biochem & Mol Biol, Stefan Meier Str 17, D-79104 Freiburg, Germany
推荐引用方式 GB/T 7714	Zhang, Kai,Zhai, Guijin,Dong, Hanyang,et al. An Efficient Approach for Selective Enrichment of Histone Modification Readers Using Self-Assembled Multivalent Photoaffinity Peptide Probes[J]. ANALYTICAL CHEMISTRY,2018,90(19):11385-11392.
APA	Zhang, Kai.,Zhai, Guijin.,Dong, Hanyang.,Guo, Zhenchang.,Feng, Wei.,...&Zhang, Yukui.(2018).An Efficient Approach for Selective Enrichment of Histone Modification Readers Using Self-Assembled Multivalent Photoaffinity Peptide Probes.ANALYTICAL CHEMISTRY,90(19),11385-11392.
MLA	Zhang, Kai,et al."An Efficient Approach for Selective Enrichment of Histone Modification Readers Using Self-Assembled Multivalent Photoaffinity Peptide Probes".ANALYTICAL CHEMISTRY 90.19(2018):11385-11392.