A 3D human lung-on-a-chip model for nanotoxicity testing

doi:10.1039/c8tx00156a

	A 3D human lung-on-a-chip model for nanotoxicity testing
	Jiang, Lei 4; Zhang, Min 4; Xu, Cong 2,4; Qin, Jianhua 1,2,3,4
刊名	TOXICOLOGY RESEARCH
	2018-11-01
卷号	7 期号:6 页码:13
ISSN号	2045-452X
DOI	10.1039/c8tx00156a
通讯作者	Qin, Jianhua(jhqin@dicp.ac.cn)
英文摘要	The prevalent application of nanoparticles (NPs) has drawn intense concerns about their impact on the environment and human health. Inhalation of NPs is the major route of NP exposure and has led to adverse effects on the lung. It is of great concern to evaluate the potential hazards of nanoparticles for human health during pulmonary exposure. Here, we proposed a novel 3D human lung-on-a-chip model to recreate the organ-level structure and functions of the human lung that allow to us evaluate the pulmonary toxicity of nanoparticles. The lung-on-a-chip consists of three parallel channels for the co-culture of human vascular endothelial cells and human alveolar epithelial cells sandwiching a layer of Matrigel membrane, which recapitulate the key features of the alveolar capillary barrier in the human lung. Cell-cell interaction, cell-matrix interaction and vascular mechanical cues work synergistically to promote the barrier function of the lung-on-a-chip model. TiO2 nanoparticles and ZnO nanoparticles were applied on the lung-on-a-chip to assay their nanotoxicity on both epithelial cells and endothelial cells. Junction protein expression, increased permeability to macromolecules, dose dependent cytotoxicity, ROS production and apoptosis were assayed and compared on the chip. This lung-on-a-chip model indicated its versatile application in human pulmonary health and safety assessment for nanoparticles, environment, food and drugs.
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDPB0305] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA16020900] ; Key Program of the Chinese Academy of Sciences[KFZD-SW-213] ; National Key R&D Program of China[2017YFB0405400] ; International Science & Technology Cooperation Program of China[2015DFA00740] ; National Nature Science Foundation of China[91543121] ; National Nature Science Foundation of China[81573394] ; National Nature Science Foundation of China[31671038] ; Innovation Program of Science and Research from the DICP, CAS[DICP TMSR201601]
WOS关键词	METAL-OXIDE NANOPARTICLES ; IN-VITRO ; ENGINEERED NANOPARTICLES ; TOXICITY ASSESSMENT ; PULMONARY TOXICITY ; EPITHELIAL-CELLS ; CELLULAR TOXICITY ; OXIDATIVE STRESS ; BARRIER FUNCTION ; SHEAR-STRESS
WOS研究方向	Toxicology
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000448823300006
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; International Science & Technology Cooperation Program of China ; International Science & Technology Cooperation Program of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; International Science & Technology Cooperation Program of China ; International Science & Technology Cooperation Program of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; International Science & Technology Cooperation Program of China ; International Science & Technology Cooperation Program of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; International Science & Technology Cooperation Program of China ; International Science & Technology Cooperation Program of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/166782]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Qin, Jianhua
作者单位	1.Chinese Acad Sci, Inst Stem Cell & Regenerat, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Shanghai, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Div Biotechnol, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Lei,Zhang, Min,Xu, Cong,et al. A 3D human lung-on-a-chip model for nanotoxicity testing[J]. TOXICOLOGY RESEARCH,2018,7(6):13.
APA	Jiang, Lei,Zhang, Min,Xu, Cong,&Qin, Jianhua.(2018).A 3D human lung-on-a-chip model for nanotoxicity testing.TOXICOLOGY RESEARCH,7(6),13.
MLA	Jiang, Lei,et al."A 3D human lung-on-a-chip model for nanotoxicity testing".TOXICOLOGY RESEARCH 7.6(2018):13.