Validation of a HRGC-ECNI/LRMS method to monitor short-chain chlorinated paraffins in human plasma

doi:10.1016/j.jes.2018.04.004

	Validation of a HRGC-ECNI/LRMS method to monitor short-chain chlorinated paraffins in human plasma
	Gao, Yuan 2; Xu, Jiazhi 2,3; Guo, Weijing 1; Wei, Linhuan 1; Zhang, Haijun 2; Zhang, Yichi 2; Sun, Ming 1; Chen, Jiping 2
刊名	JOURNAL OF ENVIRONMENTAL SCIENCES
	2019
卷号	75 页码:289-295
关键词	Short-chain chlorinated paraffins Sample preparation Quantitative analysis Human plasma
ISSN号	1001-0742
DOI	10.1016/j.jes.2018.04.004
通讯作者	Zhang, Haijun(hjzhang@dicp.ac.cn) ; Chen, Jiping(chenjp@dicp.ac.cn)
英文摘要	Short-chain chlorinated paraffins (SCCPs) are produced in high volume and have the high potential to pose a threat to human health. However, little information is available for SCCP contamination in human blood/plasma/serum, mainly due to the difficulty of sample preparation and quantitative analysis. A method using high resolution gas chromatography coupled with electron capture negative ionization low resolution mass spectrometry (HRGC-ECNI/LRMS) was developed and validated to measure SCCPs in human plasma. The pretreatment process included protein denaturation and lipid elimination, liquid-liquid extraction with a mixture of n-hexane/dichloromethane (1: 1, V/V), and cleanup on a multilayer silica column. The blank controls, including procedural blank, vacuum blood collection tube blank, and instrumental blank, were the most pivotal points for the reliable analysis of SCCPs. The average value of procedural blanks was 9.0 ng/g; and the method detection limit (MDL), calculated as the sum of the average procedural blank value and 3 times of the standard deviation of the procedural blanks, was 12.6 ng/g plasma. The validated method was applied to measure the concentrations of the total SCCPs (Sigma SCCPs) in 50 plasma samples from a general population. The measured plasma concentrations of SSCCPs ranged from
资助项目	National Natural Science Foundation of China[21337002] ; National Basic Research Program (973) of China[2015CB453100]
WOS关键词	HUMAN EXPOSURE ; FOOD-WEB ; SCCPS ; INDUCTION ; BEHAVIOR ; CHINA ; MILK ; AIR
WOS研究方向	Environmental Sciences & Ecology
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000459207900027
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program (973) of China ; National Basic Research Program (973) of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program (973) of China ; National Basic Research Program (973) of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program (973) of China ; National Basic Research Program (973) of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program (973) of China ; National Basic Research Program (973) of China
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/165956]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Zhang, Haijun; Chen, Jiping
作者单位	1.Dalian Ctr Dis Control & Prevent, Dalian 116021, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Gao, Yuan,Xu, Jiazhi,Guo, Weijing,et al. Validation of a HRGC-ECNI/LRMS method to monitor short-chain chlorinated paraffins in human plasma[J]. JOURNAL OF ENVIRONMENTAL SCIENCES,2019,75:289-295.
APA	Gao, Yuan.,Xu, Jiazhi.,Guo, Weijing.,Wei, Linhuan.,Zhang, Haijun.,...&Chen, Jiping.(2019).Validation of a HRGC-ECNI/LRMS method to monitor short-chain chlorinated paraffins in human plasma.JOURNAL OF ENVIRONMENTAL SCIENCES,75,289-295.
MLA	Gao, Yuan,et al."Validation of a HRGC-ECNI/LRMS method to monitor short-chain chlorinated paraffins in human plasma".JOURNAL OF ENVIRONMENTAL SCIENCES 75(2019):289-295.