ID4 levels dictate the stem cell state in mouse spermatogonia

	ID4 levels dictate the stem cell state in mouse spermatogonia
	Helsel, Aileen R. ; Yang, Qi-En ; Oatley, Melissa J. ; Lord, Tessa ; Sablitzky, Fred ; Oatley, Jon M.
刊名	DEVELOPMENT
	2017-02-15
英文摘要	Spermatogenesis is a classic model of cycling cell lineages that depend on a balance between stem cell self-renewal for continuity and the formation of progenitors as the initial step in the production of differentiated cells. The mechanisms that guide the continuum of spermatogonial stem cell (SSC) to progenitor spermatogonial transition and precise identifiers of subtypes in the process are undefined. Here we used an Id4-eGfp reporter mouse to discover that EGFP intensity is predictive of the subsets, with the ID4-EGFPBright population being mostly, if not purely, SSCs, whereas the ID4EGFPDim population is in transition to the progenitor state. These subsets are also distinguishable by transcriptome signatures. Moreover, using a conditional overexpression mouse model, we found that transition from the stem cell to the immediate progenitor state requires downregulation of Id4 coincident with a major change in the transcriptome. Collectively, our results demonstrate that the level of ID4 is predictive of stem cell or progenitor capacity in spermatogonia and dictates the interface of transition between the different functional states.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/6804]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Helsel, Aileen R.,Yang, Qi-En,Oatley, Melissa J.,et al. ID4 levels dictate the stem cell state in mouse spermatogonia[J]. DEVELOPMENT,2017.
APA	Helsel, Aileen R.,Yang, Qi-En,Oatley, Melissa J.,Lord, Tessa,Sablitzky, Fred,&Oatley, Jon M..(2017).ID4 levels dictate the stem cell state in mouse spermatogonia.DEVELOPMENT.
MLA	Helsel, Aileen R.,et al."ID4 levels dictate the stem cell state in mouse spermatogonia".DEVELOPMENT (2017).