Isolation of microsatellite loci for Rhodiola alsia (Crassulaceae), an important ethno-medicinal herb endemic to the Qinghai-Tibetan plateau
Zhang, F. Q. ; Lei, S. Y. ; Gao, Q. B. ; Khan, G. ; Xing, R. ; Yang, H. L. ; Chen, S. L. ; Chen, SL (reprint author), Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, Northwest Inst Plateau Biol, Xining, Peoples R China.
刊名GENETICS AND MOLECULAR RESEARCH
2015
英文摘要Rhodiola alsia, which has been used widely in traditional Chinese medicine for a considerable time, grows on moist habitats at high altitude near the snow line. Microsatellite loci were developed for R. alsia to investigate its population genetics. In total, 17 polymorphic microsatellites were developed based on ESTs from the Illumina HiSeqTM 2000 platform. The microsatellite loci were checked for variability using 80 individuals of R. alsia sampled from four locations on the Qinghai-Tibet Plateau. The total number of alleles per locus ranged from 10 to 20, and the observed heterozygosity ranged from 0.000 to 1.000. The null allele frequency ranged from 0.000 to 0.324. These microsatellites are expected to be helpful in future studies of population genetics in R. alsia and related species.; Rhodiola alsia, which has been used widely in traditional Chinese medicine for a considerable time, grows on moist habitats at high altitude near the snow line. Microsatellite loci were developed for R. alsia to investigate its population genetics. In total, 17 polymorphic microsatellites were developed based on ESTs from the Illumina HiSeqTM 2000 platform. The microsatellite loci were checked for variability using 80 individuals of R. alsia sampled from four locations on the Qinghai-Tibet Plateau. The total number of alleles per locus ranged from 10 to 20, and the observed heterozygosity ranged from 0.000 to 1.000. The null allele frequency ranged from 0.000 to 0.324. These microsatellites are expected to be helpful in future studies of population genetics in R. alsia and related species.
内容类型期刊论文
源URL[http://ir.nwipb.ac.cn/handle/363003/5475]  
专题西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Zhang, F. Q.,Lei, S. Y.,Gao, Q. B.,et al. Isolation of microsatellite loci for Rhodiola alsia (Crassulaceae), an important ethno-medicinal herb endemic to the Qinghai-Tibetan plateau[J]. GENETICS AND MOLECULAR RESEARCH,2015.
APA Zhang, F. Q..,Lei, S. Y..,Gao, Q. B..,Khan, G..,Xing, R..,...&Chen, SL .(2015).Isolation of microsatellite loci for Rhodiola alsia (Crassulaceae), an important ethno-medicinal herb endemic to the Qinghai-Tibetan plateau.GENETICS AND MOLECULAR RESEARCH.
MLA Zhang, F. Q.,et al."Isolation of microsatellite loci for Rhodiola alsia (Crassulaceae), an important ethno-medicinal herb endemic to the Qinghai-Tibetan plateau".GENETICS AND MOLECULAR RESEARCH (2015).
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