Generation of beta-lactoglobulin-modified transgenic goats by homologous recombination

	Generation of beta-lactoglobulin-modified transgenic goats by homologous recombination
	Zhu, Hongmei ; Hu, Linyong ; Liu, Jun ; Chen, Huatao ; Cui, Chenchen ; Song, Yujie ; Jin, Yaping ; Zhang, Yong
刊名	FEBS Journal
	2016-12-01
英文摘要	beta-Lactoglobulin (BLG) is a dominant allergen present in the milk of goats and other ungulates, although it is not found in human breast milk. Thus, the presence of BLG restricts the consumption of goat's milk by humans. In the present study, we examined whether the disruption of the BLG gene in goats by homologous recombination (HR) reduced BLG content in goat's milk and decreased the allergic response to milk. In one approach, exon 2 of the BLG gene was efficiently targeted using HR with a BLG knockout vector. In a second approach to disrupt BLG gene expression and drive exogenous human alpha-lactalbumin (hLA) gene expression, two hLA knock-in constructs were used to target exons 1-4 of the BLG gene via HR, and expression of hLA was then confirmed in goat mammary epithelial cells in vitro. The recombinant clones from both approaches were then used for somatic cell nuclear transfer, generating two transgenic goats possessing a BLG knockout allele or site-specific hLA integration allele. Milk assays demonstrated a reduction in BLG levels in both the BLG knockout and hLA knock-in goats; furthermore, hLA was present in the hLA knock-in goat's milk. Allergenic analysis in mice indicated that the transgenic goat's milk was less allergenic than wild-type goat's milk. These results support the development of gene-targeted animals as an effective tool for reducing allergic reactions to milk and improving nutrition.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/6780]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhu, Hongmei,Hu, Linyong,Liu, Jun,et al. Generation of beta-lactoglobulin-modified transgenic goats by homologous recombination[J]. FEBS Journal,2016.
APA	Zhu, Hongmei.,Hu, Linyong.,Liu, Jun.,Chen, Huatao.,Cui, Chenchen.,...&Zhang, Yong.(2016).Generation of beta-lactoglobulin-modified transgenic goats by homologous recombination.FEBS Journal.
MLA	Zhu, Hongmei,et al."Generation of beta-lactoglobulin-modified transgenic goats by homologous recombination".FEBS Journal (2016).