Isolation and Purification of Three Analogues from Clematis akebioides by Molecularly Imprinted Solid-Phase Extraction and HSCCC

	Isolation and Purification of Three Analogues from Clematis akebioides by Molecularly Imprinted Solid-Phase Extraction and HSCCC
	Liu, Hongliu ; Gong, Chengcheng ; Liao, Zhixin ; Chen, Tao ; Li, Yulin
刊名	CHROMATOGRAPHIA
	2017-11-01
英文摘要	An efficient strategy of using molecularly imprinted solid-phase extraction and high-speed counter-current chromatography (HSCCC) were established for separation and purification of three analogues from Clematis akebioides for the first time. The three analogues, including 26.30 mg caffeic acid, 84.20 mg p-coumaric acid, and 44.40 mg ferulic acid, were obtained from 200.00 mg of the crude sample. The molecularly imprinted polymer was prepared with caffeic acid as template molecule and acrylamide as functional monomers. The results showed that the caffeic acid and its analogues could be well enriched. Then, the extract was purified by HSCCC using a two-phase solvent system consisting of chloroform/acetonitrile/water/acetic acid (10:5:4:0.0004, v/v) and a continuous sample introduction system was adopted. The three organic acids were identified by H-1-NMR and C-13-NMR.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/10361]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Liu, Hongliu,Gong, Chengcheng,Liao, Zhixin,et al. Isolation and Purification of Three Analogues from Clematis akebioides by Molecularly Imprinted Solid-Phase Extraction and HSCCC[J]. CHROMATOGRAPHIA,2017.
APA	Liu, Hongliu,Gong, Chengcheng,Liao, Zhixin,Chen, Tao,&Li, Yulin.(2017).Isolation and Purification of Three Analogues from Clematis akebioides by Molecularly Imprinted Solid-Phase Extraction and HSCCC.CHROMATOGRAPHIA.
MLA	Liu, Hongliu,et al."Isolation and Purification of Three Analogues from Clematis akebioides by Molecularly Imprinted Solid-Phase Extraction and HSCCC".CHROMATOGRAPHIA (2017).