Determination of Fatty Acids (C-1-C-10) from Bryophytes and Pteridophytes

	Determination of Fatty Acids (C-1-C-10) from Bryophytes and Pteridophytes
	Bai, Xin-Wei ; Song, Cui-Hua ; You, Jin-Mao ; Sun, Zhi-Wei ; Fu, Yan-Yan ; Li, Guo-Liang
刊名	CHROMATOGRAPHIA
	2010-06-01
英文摘要	A simple and mild method for the determination of fatty acids (C-1 - C-10) based on a condensation reaction using 7-aminonaphthalene-1,3-disulfonic acid (ANDSA) as labeling reagent with capillary zone electrophoresis has been developed. The detection was performed with a diode array detector at 254 nm. A 58.5 cm x 50 mu m i.d. (50 cm effective length) untreated fused-silica capillary was used. To optimize the separation conditions, the background electrolyte concentration, column temperature, voltage and other factors were evaluated. The optimal separation conditions were as follows: 30 mmol L-1 borate buffer (pH 9.5), 15 mmol L-1 beta-CD, temperature at 20 A degrees C, pressure 50 mbar and injection time 8 s. Under the established conditions, 10 fatty acid derivatives could be well-separated within 17 min. The linearity was in the range of 0.07-5.0 mu mol L-1. Detection limits (at a signal-to-noise ratio of 3) were in the range of 0.027-0.042 mu mol L-1. The fatty acids from the extracted Funaria Hedw. and Selaginella samples were determined with satisfactory results.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/6888]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Bai, Xin-Wei,Song, Cui-Hua,You, Jin-Mao,et al. Determination of Fatty Acids (C-1-C-10) from Bryophytes and Pteridophytes[J]. CHROMATOGRAPHIA,2010.
APA	Bai, Xin-Wei,Song, Cui-Hua,You, Jin-Mao,Sun, Zhi-Wei,Fu, Yan-Yan,&Li, Guo-Liang.(2010).Determination of Fatty Acids (C-1-C-10) from Bryophytes and Pteridophytes.CHROMATOGRAPHIA.
MLA	Bai, Xin-Wei,et al."Determination of Fatty Acids (C-1-C-10) from Bryophytes and Pteridophytes".CHROMATOGRAPHIA (2010).