One Step to Separate Five Alkaloids from Hypecoum leptocarpum by High-Speed Counter-Current Chromatography

	One Step to Separate Five Alkaloids from Hypecoum leptocarpum by High-Speed Counter-Current Chromatography
	Zhang, Qiulong ; Ma, Tao ; Hu, Na ; Ding, Chenxi ; Liang, Yongxin ; Li, Wencong ; Suo, Yourui ; Ding, Chenxu
刊名	JOURNAL OF CHROMATOGRAPHIC SCIENCE
	2016-03-01
英文摘要	High-speed counter-current chromatography (CCC) was firstly and successfully applied for the preparative separation and purification of alkaloids from crude extract of Hypecoum leptocarpum. After the measurement of partition coefficient of five target alkaloids in the two-phase solvent systems, the CCC was performed well with a two-phase solvent system composed of tetrachloromethane-chloroform-methanol-0.1 M HCl at a volume ratio of 1.5 : 2.5 : 3 : 2 (V/V/V/V). The upper phase was used as the stationary phase, and the lower phasewas used as the mobile phase. From 120 mg crude extract, 5 mg leptopidine, 32 mg oxohydrastinine, 27 mg (-)-N-methylanadine, 7 mg N-feruloyltyramine and 3 mg hypecoleptopine could be successfully separated. The amides alkaloid, N-feruloyltyramine, was firstly separated from H. leptocarpum. High-performance liquid chromatography analysis showed that the purity of each of the five target alkaloids was over 92%. Their chemical structures were confirmed by H-1-NMR and C-13-NMR data.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/6465]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhang, Qiulong,Ma, Tao,Hu, Na,et al. One Step to Separate Five Alkaloids from Hypecoum leptocarpum by High-Speed Counter-Current Chromatography[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2016.
APA	Zhang, Qiulong.,Ma, Tao.,Hu, Na.,Ding, Chenxi.,Liang, Yongxin.,...&Ding, Chenxu.(2016).One Step to Separate Five Alkaloids from Hypecoum leptocarpum by High-Speed Counter-Current Chromatography.JOURNAL OF CHROMATOGRAPHIC SCIENCE.
MLA	Zhang, Qiulong,et al."One Step to Separate Five Alkaloids from Hypecoum leptocarpum by High-Speed Counter-Current Chromatography".JOURNAL OF CHROMATOGRAPHIC SCIENCE (2016).