DETERMINATION OF FREE FATTY ACIDS IN CORTEX LYCII BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROMETRY WITH FLUORESCENCE DETECTION AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION IDENTIFICATION

	DETERMINATION OF FREE FATTY ACIDS IN CORTEX LYCII BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROMETRY WITH FLUORESCENCE DETECTION AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION IDENTIFICATION
	Chen, Xiangming ; Dong, Xiuli ; Suo, Yourui ; You, Jinmao
刊名	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES ; Chen, XM; Dong, XL; Suo, YR; You, JM.DETERMINATION OF FREE FATTY ACIDS IN CORTEX LYCII BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROMETRY WITH FLUORESCENCE DETECTION AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION IDENTIFICATION,JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2014,37(11):1548
	2014-07-03
英文摘要	A highly sensitive method for the determination of free fatty acids (FFAs) using high-performance liquid chromatography (HPLC) with fluorescence detection has been developed. Twenty-six FFAs (C5-C30) were derivatized by a novel pre-column derivatization reagent 2-(11H-benzo[a]carbazol-11-yl)-ethyl-4-methyl- benzenesulphonate (BCETS). Derivatives were sufficiently stable to be efficiently analyzed by HPLC. All fatty acid derivatives were separated on a hypersil BDS-C8 column in conjunction with a gradient elution with a good baseline resolution. The identification was carried out by post-column online mass spectrometry with atmospheric pressure chemical ionization (APCI) in positive-ion detection mode. The established method exhibited good selectivity and excellent repeatability. The RSD (n=6) for each fatty acid derivative was <2.8%, and detection limit (at signal-to-noise of 3:1) was 15-64 fmol. The method was used to quantify FFAs in Cortex Lycii sample with satisfactory results.; A highly sensitive method for the determination of free fatty acids (FFAs) using high-performance liquid chromatography (HPLC) with fluorescence detection has been developed. Twenty-six FFAs (C5-C30) were derivatized by a novel pre-column derivatization reagent 2-(11H-benzo[a]carbazol-11-yl)-ethyl-4-methyl- benzenesulphonate (BCETS). Derivatives were sufficiently stable to be efficiently analyzed by HPLC. All fatty acid derivatives were separated on a hypersil BDS-C8 column in conjunction with a gradient elution with a good baseline resolution. The identification was carried out by post-column online mass spectrometry with atmospheric pressure chemical ionization (APCI) in positive-ion detection mode. The established method exhibited good selectivity and excellent repeatability. The RSD (n=6) for each fatty acid derivative was <2.8%, and detection limit (at signal-to-noise of 3:1) was 15-64 fmol. The method was used to quantify FFAs in Cortex Lycii sample with satisfactory results.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/4210]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Chen, Xiangming,Dong, Xiuli,Suo, Yourui,et al. DETERMINATION OF FREE FATTY ACIDS IN CORTEX LYCII BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROMETRY WITH FLUORESCENCE DETECTION AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION IDENTIFICATION[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, Chen, XM; Dong, XL; Suo, YR; You, JM.DETERMINATION OF FREE FATTY ACIDS IN CORTEX LYCII BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROMETRY WITH FLUORESCENCE DETECTION AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION IDENTIFICATION,JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2014,37(11):1548,2014.
APA	Chen, Xiangming,Dong, Xiuli,Suo, Yourui,&You, Jinmao.(2014).DETERMINATION OF FREE FATTY ACIDS IN CORTEX LYCII BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROMETRY WITH FLUORESCENCE DETECTION AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION IDENTIFICATION.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES.
MLA	Chen, Xiangming,et al."DETERMINATION OF FREE FATTY ACIDS IN CORTEX LYCII BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROMETRY WITH FLUORESCENCE DETECTION AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION IDENTIFICATION".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES (2014).