Structure and evolutionary relationships among paralogous genes within the Sec2 locus in rye

	Structure and evolutionary relationships among paralogous genes within the Sec2 locus in rye
	Wang, Yanqian ; Zhang, Bo ; Liu, Baolong ; Zhang, Huaigang ; Liu, Dengcai
刊名	JOURNAL OF CEREAL SCIENCE ; Zhang, BY; Wang, H; Luo, XD; Du, ZZ; Shen, JW; Wu, HF; Zhang, XF.Structure and evolutionary relationships among paralogous genes within the Sec2 locus in rye,JOURNAL OF CEREAL SCIENCE,2012,56(2):282-288
	2012-09-01
英文摘要	The 75K gamma-secalins encoded by genes present at the locus Sec2 on chromosome 2R are unique to rye and contribute about half of all rye storage proteins. However, there is a lack of sequence information for paralogous genes in this locus. For this study, 59 gamma-secalin paralogous sequences in the Sec2 locus were characterized from a cultivated rye and derived lines after crossing with bread wheat. They had similar structures with conserved sequences in their repetitive regions for the signal peptide, N-terminal, C-terminal and the repeat motif. Their high homology indicated that they originated from an ancestor sequence that existed before the speciation of the genus Secale. Duplication and divergence might have led to the formation of the paralogous genes at Sec2. Besides point mutations, these paralogs showed variations in DNA length due to insertion or deletion events in their repetitive regions. They encoded secalins with deduced molecular weight ranges between 22.2 and 54.5 kDa. These insertion or deletions may be caused by illegitimate recombination and this locus seemed to contribute to increased levels of protein content. However, the incorporation of locus Sec2 may have a negative effect on flour processing quality since it reduced the SDS-sedimentation value. (C) 2012 Elsevier Ltd. All rights reserved.; The 75K gamma-secalins encoded by genes present at the locus Sec2 on chromosome 2R are unique to rye and contribute about half of all rye storage proteins. However, there is a lack of sequence information for paralogous genes in this locus. For this study, 59 gamma-secalin paralogous sequences in the Sec2 locus were characterized from a cultivated rye and derived lines after crossing with bread wheat. They had similar structures with conserved sequences in their repetitive regions for the signal peptide, N-terminal, C-terminal and the repeat motif. Their high homology indicated that they originated from an ancestor sequence that existed before the speciation of the genus Secale. Duplication and divergence might have led to the formation of the paralogous genes at Sec2. Besides point mutations, these paralogs showed variations in DNA length due to insertion or deletion events in their repetitive regions. They encoded secalins with deduced molecular weight ranges between 22.2 and 54.5 kDa. These insertion or deletions may be caused by illegitimate recombination and this locus seemed to contribute to increased levels of protein content. However, the incorporation of locus Sec2 may have a negative effect on flour processing quality since it reduced the SDS-sedimentation value. (C) 2012 Elsevier Ltd. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/3610]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, Yanqian,Zhang, Bo,Liu, Baolong,et al. Structure and evolutionary relationships among paralogous genes within the Sec2 locus in rye[J]. JOURNAL OF CEREAL SCIENCE, Zhang, BY; Wang, H; Luo, XD; Du, ZZ; Shen, JW; Wu, HF; Zhang, XF.Structure and evolutionary relationships among paralogous genes within the Sec2 locus in rye,JOURNAL OF CEREAL SCIENCE,2012,56(2):282-288,2012.
APA	Wang, Yanqian,Zhang, Bo,Liu, Baolong,Zhang, Huaigang,&Liu, Dengcai.(2012).Structure and evolutionary relationships among paralogous genes within the Sec2 locus in rye.JOURNAL OF CEREAL SCIENCE.
MLA	Wang, Yanqian,et al."Structure and evolutionary relationships among paralogous genes within the Sec2 locus in rye".JOURNAL OF CEREAL SCIENCE (2012).