| High-performance affinity monolith chromatography for chiral separation and determination of enzyme kinetic constants | |
| Yao, Chunhe1,3; Qi, Li1; Qiao, Juan1,3; Zhang, Haizhi1,3; Wang, Fuyi1; Chen, Yi1; Yang, Gengliang2 | |
| 刊名 | Talanta
 |
| 2010-09-15 | |
| 卷号 | 82期号:4页码:1332-1337 |
| 关键词 | High-performance affinity chromatography Human serum albumin Enzyme kinetic study Chiral separation Monolithic column |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2010.06.041 |
| 通讯作者 | Qi, li(qili@iccas.ac.cn) |
| 英文摘要 | A new kind of immobilized human serum albumin (hsa) column was developed by using the sub-micron skeletal polymer monolith based on poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) [poly(gma-edma)] as the support of high-performance affinity chromatography. using the epoxide functional groups presented in gma, the hsa immobilization procedure was performed by two different means. the affinity columns were successfully adopted for the chiral separation of d,l-amino acids (aas). then this method was shown to be applicable to the quantitative analysis of d-tryptophan, with a linear ;range between 12.0 mu m and 979.0 mu m, and a correlation coefficient above 0.99. furthermore, it was used for the analysis of urine sample. this assay is demonstrated to be facile and relatively rapid. so it allows us to measure the enzyme catalytic activity in the incubation of d,l-aas with d-aa oxidase and to study the kinetics of the enzyme reaction. it implied that the affinity monolithic columns can be a useful tool for studying daao enzyme reaction and investigating the potential enzyme mechanism requirement among chiral conversion. (c) 2010 elsevier b.v. all rights reserved. |
| WOS关键词 | HUMAN SERUM-ALBUMIN ; AMINO-ACID OXIDASE ; LIQUID-CHROMATOGRAPHY ; CAPILLARY-ELECTROPHORESIS ; STATIONARY-PHASE ; DANSYL AMINO ; POLYMERS ; COLUMNS ; ENANTIOMERS ; SUBSTRATE |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000282480500036 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2410974 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Qi, Li |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Hebei Univ, Coll Pharm, Baoding 071002, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yao, Chunhe,Qi, Li,Qiao, Juan,et al. High-performance affinity monolith chromatography for chiral separation and determination of enzyme kinetic constants[J]. Talanta,2010,82(4):1332-1337. |
| APA | Yao, Chunhe.,Qi, Li.,Qiao, Juan.,Zhang, Haizhi.,Wang, Fuyi.,...&Yang, Gengliang.(2010).High-performance affinity monolith chromatography for chiral separation and determination of enzyme kinetic constants.Talanta,82(4),1332-1337. |
| MLA | Yao, Chunhe,et al."High-performance affinity monolith chromatography for chiral separation and determination of enzyme kinetic constants".Talanta 82.4(2010):1332-1337. |
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