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	Arginine methylation mediated by the arabidopsis homolog of prmt5 is essential for proper pre-mrna splicing
	Deng, Xian1,2,3; Gu, Lianfeng1,2,3; Liu, Chunyan1,2; Lu, Tiancong1,2,3,4; Lu, Falong1,2,3; Lu, Zhike1,2; Cui, Peng5; Pei, Yanxi1,2,6; Wang, Baichen4; Hu, Songnian5
刊名	Proceedings of the national academy of sciences of the united states of america
	2010-11-02
卷号	107期号:44页码:19114-19119
ISSN号	0027-8424
DOI	10.1073/pnas.1009669107
通讯作者	Cao, xiaofeng(xfcao@genetics.ac.cn)
英文摘要	Protein arginine methylation, one of the most abundant and important posttranslational modifications, is involved in a multitude of biological processes in eukaryotes, such as transcriptional regulation and rna processing. symmetric arginine dimethylation is required for snrnp biogenesis and is assumed to be essential for pre-mrna splicing; however, except for in vitro evidence, whether it affects splicing in vivo remains elusive. mutation in an arabidopsis symmetric arginine dimethyltransferase, atprmt5, causes pleiotropic developmental defects, including late flowering, but the underlying molecular mechanism is largely unknown. here we show that atprmt5 methylates a wide spectrum of substrates, including some rna binding or processing factors and u snrnp atsmd1, d3, and atlsm4 proteins, which are involved in rna metabolism. rna-seq analyses reveal that atprmt5 deficiency causes splicing defects in hundreds of genes involved in multiple biological processes. the splicing defects are identified in transcripts of several rna processing factors involved in regulating flowering time. in particular, splicing defects at the flowering regulator flowering locus kh domain (flk) in atprmt5 mutants reduce its functional transcript and protein levels, resulting in the up-regulation of a flowering repressor flowering locus c (flc) and consequently late flowering. taken together, our findings uncover an essential role for arginine methylation in proper pre-mrna splicing that impacts diverse developmental processes.
WOS关键词	FLOWERING TIME ; BINDING PROTEINS ; SM PROTEINS ; PROTEOMIC ANALYSIS ; REPEAT PROTEIN ; METHYLTRANSFERASE ; THALIANA ; COMPLEX ; GENE ; DIMETHYLATION
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
语种	英语
出版者	NATL ACAD SCIENCES
WOS记录号	WOS:000283749000068
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2409882
专题	中国科学院大学
通讯作者	Cao, Xiaofeng
作者单位	1.Chinese Acad Sci, State Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Natl Ctr Plant Gene Res, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 4.NE Forestry Univ, Harbin 150040, Peoples R China 5.Chinese Acad Sci, Beijing Inst Genom, Beijing 100029, Peoples R China 6.Shanxi Univ, Coll Life Sci & Technol, Taiyuan 030006, Peoples R China
推荐引用方式 GB/T 7714	Deng, Xian,Gu, Lianfeng,Liu, Chunyan,et al. Arginine methylation mediated by the arabidopsis homolog of prmt5 is essential for proper pre-mrna splicing[J]. Proceedings of the national academy of sciences of the united states of america,2010,107(44):19114-19119.
APA	Deng, Xian.,Gu, Lianfeng.,Liu, Chunyan.,Lu, Tiancong.,Lu, Falong.,...&Cao, Xiaofeng.(2010).Arginine methylation mediated by the arabidopsis homolog of prmt5 is essential for proper pre-mrna splicing.Proceedings of the national academy of sciences of the united states of america,107(44),19114-19119.
MLA	Deng, Xian,et al."Arginine methylation mediated by the arabidopsis homolog of prmt5 is essential for proper pre-mrna splicing".Proceedings of the national academy of sciences of the united states of america 107.44(2010):19114-19119.

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