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	Subcellular localization and topology of porcine reproductive and respiratory syndrome virus e protein
	Yu, Maorong1,2; Liu, Xiaoling1,2; Sun, Lei1,2; Chen, Caiwei1,2; Ma, Guangpeng3; Kitamura, Yoshihiro4; Gao, George F.1,2,4; Liu, Wenjun1,2,4
刊名	Virus research
	2010-09-01
卷号	152期号:1-2页码:104-114
关键词	Subcellular localization Topology Prrs virus Envelope protein e Membrane association
ISSN号	0168-1702
DOI	10.1016/j.virusres.2010.06.012
通讯作者	Liu, wenjun(liuwj@im.ac.cn)
英文摘要	Porcine reproductive and respiratory syndrome virus (prrsv) open reading frame (orf) 2a contains a small internal orf (2b) capable of encoding a protein of 70 or 73 amino acids (aa), termed e protein. the function and biochemical information of the e protein are currently not clear. in the present investigation, it was shown that the e protein was mainly located in the endoplasmic reticulum (er) and golgi complex in marc-145 cells. deletion studies identified the n-terminal 15 residues as an er localization sequence of the e protein, besides two other localization sequences within positions 23-50 and 50-73, and the n-myristoylation site significantly affected the subcellular localization of the n-terminal 15 residues. the membrane association assay demonstrated that the e protein was an integral membrane protein embedded in the phospholipid bilayer. however, neither the n-myristoylation site nor the hydrophilic c-terminal domain was essential to the membrane association of the e protein. the topology analysis revealed that this protein had n-terminus oriented toward the cytoplasm and c-terminus toward the er lumen. finally, immunofluorescence assay indicated that the e protein colocalized with gp2, gp3, gp4 and m protein in cotransfected cells, but not n protein. (c) 2010 elsevier b.v. all rights reserved.
WOS关键词	MEMBRANE ASSOCIATION ; STRUCTURAL PROTEIN ; ENVELOPE PROTEIN ; LELYSTAD-VIRUS ; IDENTIFICATION ; ARTERIVIRUSES ; MACROPHAGES
WOS研究方向	Virology
WOS类目	Virology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000281360300013
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2408827
专题	中国科学院大学
通讯作者	Liu, Wenjun
作者单位	1.Chinese Acad Sci, Inst Microbiol, Ctr Mol Virol, Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100101, Peoples R China 3.China Rural Technol Dev Ctr, Beijing 100045, Peoples R China 4.Chinese Acad Sci, Inst Microbiol, China Japan Joint Lab Mol Immunol & Mol Microbiol, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Yu, Maorong,Liu, Xiaoling,Sun, Lei,et al. Subcellular localization and topology of porcine reproductive and respiratory syndrome virus e protein[J]. Virus research,2010,152(1-2):104-114.
APA	Yu, Maorong.,Liu, Xiaoling.,Sun, Lei.,Chen, Caiwei.,Ma, Guangpeng.,...&Liu, Wenjun.(2010).Subcellular localization and topology of porcine reproductive and respiratory syndrome virus e protein.Virus research,152(1-2),104-114.
MLA	Yu, Maorong,et al."Subcellular localization and topology of porcine reproductive and respiratory syndrome virus e protein".Virus research 152.1-2(2010):104-114.

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