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	Identification of immune-relevant genes by expressed sequence tag analysis of head kidney from grass carp (ctenopharyngodon idella)
	Liu, Feng2; Wang, Dapeng1,3; Fu, Jianjun2; Sun, Gaoyuan1; Shen, Yubang2; Dong, Lingli1; Zhang, Bing1; Hu, Songnian1; Li, Jiale2,4
刊名	Comparative biochemistry and physiology d-genomics & proteomics
	2010-06-01
卷号	5期号:2页码:116-123
关键词	Grass carp Cdna library Head kidney Immune-related gene Fcr gamma Immunoglobulin light chains
ISSN号	1744-117X
DOI	10.1016/j.cbd.2010.02.003
通讯作者	Hu, songnian(husn@big.ac.cn)
英文摘要	Grass carp is the third largest aquaculture species in global production. however, genomic research of this species has been limited. to identify immune-related genes in grass carp, a normalized full-length cdna library was constructed from head kidney tissues, and 6432 randomly selected clones were sequenced. 5289 high quality expressed sequence tags (est) were generated and assembled into 2687 unigenes. among them, 1585 unigenes showed significant similarity with known sequences in public databases, whereas the remaining 1102 unigenes appeared to be novel sequences with unknown functions. in particular, 136 immune-related genes were identified to encode immunoglobulins, fcr gamma, ifn-related proteins, various cd markers, mhcs, complements and other important immune-related factors: a majority of these genes are reported in grass carp for the first time. sequence analysis indicated that grass carp has at least three subtypes of immunoglobulin light chains, namely l1, l2 and l3. furthermore, fcr gamma was found to broadly express in different tissues. our study constitutes the first est analysis of lymphatic tissue in grass carp, and could pave the way for further research of immune-related genes and functional genomics in grass carp. crown copyright (c) 2010 published by elsevier inc. all rights reserved.
WOS关键词	LIGHT-CHAIN ISOTYPES ; CDNA LIBRARY ; GENOMIC ORGANIZATION ; MOLECULAR-CLONING ; CHANNEL CATFISH ; COMMON CARP ; IMMUNOGLOBULIN ; VERTEBRATES ; SYSTEM ; CELLS
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS类目	Biochemistry & Molecular Biology ; Genetics & Heredity
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000279214000005
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2408816
专题	中国科学院大学
通讯作者	Hu, Songnian
作者单位	1.Chinese Acad Sci, Beijing Inst Genom, Key Lab Genome Sci & Informat, Beijing 100029, Peoples R China 2.Shanghai Ocean Univ, Minist Educ, Key Lab Explorat & Utilizat Aquat Genet Resources, Shanghai 201306, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Shanghai Ocean Univ, E Inst Shanghai Univ, Aquaculture Div, Shanghai 201306, Peoples R China
推荐引用方式 GB/T 7714	Liu, Feng,Wang, Dapeng,Fu, Jianjun,et al. Identification of immune-relevant genes by expressed sequence tag analysis of head kidney from grass carp (ctenopharyngodon idella)[J]. Comparative biochemistry and physiology d-genomics & proteomics,2010,5(2):116-123.
APA	Liu, Feng.,Wang, Dapeng.,Fu, Jianjun.,Sun, Gaoyuan.,Shen, Yubang.,...&Li, Jiale.(2010).Identification of immune-relevant genes by expressed sequence tag analysis of head kidney from grass carp (ctenopharyngodon idella).Comparative biochemistry and physiology d-genomics & proteomics,5(2),116-123.
MLA	Liu, Feng,et al."Identification of immune-relevant genes by expressed sequence tag analysis of head kidney from grass carp (ctenopharyngodon idella)".Comparative biochemistry and physiology d-genomics & proteomics 5.2(2010):116-123.

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