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	Massively parallel pyrosequencing-based transcriptome analyses of small brown planthopper (laodelphax striatellus), a vector insect transmitting rice stripe virus (rsv)
	Zhang, Fujie1,2,3; Guo, Hongyan1,2,3; Zheng, Huajun4; Zhou, Tong5; Zhou, Yijun5; Wang, Shengyue4; Fang, Rongxiang1,2; Qian, Wei1,2; Chen, Xiaoying1,2
刊名	Bmc genomics
	2010-05-13
卷号	11页码:13
ISSN号	1471-2164
DOI	10.1186/1471-2164-11-303
通讯作者	Qian, wei(qianw@im.ac.cn)
英文摘要	Background: the small brown planthopper (laodelphax striatellus) is an important agricultural pest that not only damages rice plants by sap-sucking, but also acts as a vector that transmits rice stripe virus (rsv), which can cause even more serious yield loss. despite being a model organism for studying entomology, population biology, plant protection, molecular interactions among plants, viruses and insects, only a few genomic sequences are available for this species. to investigate its transcriptome and determine the differences between viruliferous and nave l. striatellus, we employed 454-flx high-throughput pyrosequencing to generate est databases of this insect. results: we obtained 201,281 and 218,681 high-quality reads from viruliferous and nave l. striatellus, respectively, with an average read length as 230 bp. these reads were assembled into contigs and two est databases were generated. when all reads were combined, 16,885 contigs and 24,607 singletons (a total of 41,492 unigenes) were obtained, which represents a transcriptome of the insect. blastx search against the ncbi-nr database revealed that only 6,873 (16.6%) of these unigenes have significant matches. comparison of the distribution of go classification among viruliferous, nave, and combined est databases indicated that these libraries are broadly representative of the l. striatellus transcriptomes. functionally diverse transcripts from rsv, endosymbiotic bacteria wolbachia and yeast-like symbiotes were identified, which reflects the possible lifestyles of these microbial symbionts that live in the cells of the host insect. comparative genomic analysis revealed that l. striatellus encodes similar innate immunity regulatory systems as other insects, such as rna interference, jak/stat and partial imd cascades, which might be involved in defense against viral infection. in addition, we determined the differences in gene expression between vector and nave samples, which generated a list of candidate genes that are potentially involved in the symbiosis of l. striatellus and rsv. conclusions: to our knowledge, the present study is the first description of a genomic project for l. striatellus. the identification of transcripts from rsv, wolbachia, yeast-like symbiotes and genes abundantly expressed in viruliferous insect, provided a starting-point for investigating the molecular basis of symbiosis among these organisms.
WOS关键词	DROSOPHILA IMD PATHWAY ; ENDOGENOUS RETROVIRUS ; IMMUNE-RESPONSES ; PLANT-VIRUSES ; HOST-DEFENSE ; MELANOGASTER ; WOLBACHIA ; RECOGNITION ; BIOLOGY ; IRON
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000279862300001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2407828
专题	中国科学院大学
通讯作者	Qian, Wei
作者单位	1.Chinese Acad Sci, Inst Microbiol, State Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Natl Plant Gene Res Ctr, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 4.Chinese Natl Human Genome Ctr Shanghai, Shanghai MOST Key Lab Hlth & Dis Genom, Shanghai 200032, Peoples R China 5.Jiangsu Acad Agr Sci, Nanjing 210014, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Fujie,Guo, Hongyan,Zheng, Huajun,et al. Massively parallel pyrosequencing-based transcriptome analyses of small brown planthopper (laodelphax striatellus), a vector insect transmitting rice stripe virus (rsv)[J]. Bmc genomics,2010,11:13.
APA	Zhang, Fujie.,Guo, Hongyan.,Zheng, Huajun.,Zhou, Tong.,Zhou, Yijun.,...&Chen, Xiaoying.(2010).Massively parallel pyrosequencing-based transcriptome analyses of small brown planthopper (laodelphax striatellus), a vector insect transmitting rice stripe virus (rsv).Bmc genomics,11,13.
MLA	Zhang, Fujie,et al."Massively parallel pyrosequencing-based transcriptome analyses of small brown planthopper (laodelphax striatellus), a vector insect transmitting rice stripe virus (rsv)".Bmc genomics 11(2010):13.

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