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	Conserved mirna analysis in gossypium hirsutum through small rna sequencing
	Ruan, Meng-Bin2; Zhao, Ying-Tao1,3; Meng, Zhao-Hong2; Wang, Xiu-Jie1; Yang, Wei-Cai2
刊名	Genomics
	2009-10-01
卷号	94期号:4页码:263-268
关键词	Gossypium hirsutum Mirna Identification Sequencing
ISSN号	0888-7543
DOI	10.1016/j.ygeno.2009.07.002
通讯作者	Wang, xiu-jie(xjwang@genetics.ac.cn)
英文摘要	Several mirna family and their targets in cotton had been identified by computational methods based on the conserved characterization of mirnas. so far, there are no experiments to validate the existence of mirnas in cotton. in this study, to analyze the mirnas in cotton, a small rna library of sequences from 18 to 26 nt of gossypium hirsutum seedling has been built by high-throughput sequencing. in this library, 34 conserved mirna families were identified by homology search and the mirna* sequences of them were also found in the library. furthermore, potential targets of these conserved mirna families were predicted in cotton tc library. however, based on the mature mirnas and their mir* sequences, only 8 conserved mirna encoding loci (mir156, mir157a, mir157b, mir162, mir164, mir393, mir399, mir827) were identified from cotton est sequences. multiple encoding loci of some mirnas were identified by comparing the cloned mirna and mir* sequences. (c) 2009 elsevier inc. all rights reserved.
WOS关键词	LATERAL ROOT DEVELOPMENT ; MESSENGER-RNA ; LEAF POLARITY ; CELL FATE ; MICRORNA ; PLANT ; ARABIDOPSIS ; IDENTIFICATION ; COTTON ; EVOLUTION
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000273387100007
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2401049
专题	中国科学院大学
通讯作者	Wang, Xiu-Jie
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Gen, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res Beijing, Key Lab Mol & Dev Biol, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Ruan, Meng-Bin,Zhao, Ying-Tao,Meng, Zhao-Hong,et al. Conserved mirna analysis in gossypium hirsutum through small rna sequencing[J]. Genomics,2009,94(4):263-268.
APA	Ruan, Meng-Bin,Zhao, Ying-Tao,Meng, Zhao-Hong,Wang, Xiu-Jie,&Yang, Wei-Cai.(2009).Conserved mirna analysis in gossypium hirsutum through small rna sequencing.Genomics,94(4),263-268.
MLA	Ruan, Meng-Bin,et al."Conserved mirna analysis in gossypium hirsutum through small rna sequencing".Genomics 94.4(2009):263-268.

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