QQ客服 官方微博 反馈留言

	Molecular analysis of rice plants harboring a multi-functional t-dna tagging system
	Ma, Yimian1,2,3; Liu, Luo1,2; Zhu, Chengguang1,2; Sun, Changhui1,2; Xu, Bo1,2,3; Fang, Jun1,2; Tang, Jiuyou1,2,3; Luo, Anding1,2; Cao, Shouyun1,2; Li, Gupo1,2
刊名	Journal of genetics and genomics
	2009-05-01
卷号	36期号:5页码:267-276
关键词	Rice Mutant population Promoter trap Activation tag T-dna integration
ISSN号	1673-8527
DOI	10.1016/s1673-8527(08)60114-9
通讯作者	Chu, chengcai(ccchu@genetics.ac.cn)
英文摘要	About 25,000 rice t-dna insertional mutant lines were generated using the vector pcas04 which has both promoter-trapping and activation-tagging function. southern blot analysis revealed that about 40% of these mutants were single copy integration and the average t-dna insertion number was 2.28. by extensive phenotyping in the field, quite a number of agronomically important mutants were obtained. histochemical gus assay with 4,3 10 primary mutants revealed that the gus-staining frequency was higher than that of the previous reports in various tissues and especially high in flowers. the t-dna flanking sequences of some mutants were isolated and the t-dna insertion sites were mapped to the rice genome. the flanking sequence analysis demonstrated the different integration pattern of the right border and left border into rice genome. compared with arabidopsis and poplar, it is much varied in the t-dna border junctions in rice.
WOS关键词	ARABIDOPSIS-THALIANA ; FUNCTIONAL GENOMICS ; ILLEGITIMATE RECOMBINATION ; INSERTIONAL MUTAGENESIS ; TRANSGENE EXPRESSION ; TRAPPING SYSTEM ; DRAFT SEQUENCE ; PROMOTER TRAP ; BINARY VECTOR ; ACTIVATION
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS类目	Biochemistry & Molecular Biology ; Genetics & Heredity
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000266053600001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2398591
专题	中国科学院大学
通讯作者	Chu, Chengcai
作者单位	1.Chinese Acad Sci, State Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Natl Plant Gene Res Ctr Beijing, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China 4.Chinese Acad Agr Sci, China Inst Rice Res, State Key Lab Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China 5.Chinese Acad Sci, Inst Genet & Dev Biol, Mol & Dev Biol Lab, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Ma, Yimian,Liu, Luo,Zhu, Chengguang,et al. Molecular analysis of rice plants harboring a multi-functional t-dna tagging system[J]. Journal of genetics and genomics,2009,36(5):267-276.
APA	Ma, Yimian.,Liu, Luo.,Zhu, Chengguang.,Sun, Changhui.,Xu, Bo.,...&Chu, Chengcai.(2009).Molecular analysis of rice plants harboring a multi-functional t-dna tagging system.Journal of genetics and genomics,36(5),267-276.
MLA	Ma, Yimian,et al."Molecular analysis of rice plants harboring a multi-functional t-dna tagging system".Journal of genetics and genomics 36.5(2009):267-276.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。