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	Ipt gene transformation in petunia by an agrobacterium mediated method
	Bai, L. J.1,2; Ye, C. J.1; Lu, J. Y.1; Yang, D. E.1; Xue, H.1; Pan, Y.1; Cao, P. X.1; Wang, B.1; Liu, M.1
刊名	Journal of immunoassay & immunochemistry
	2009
卷号	30期号:2页码:224-231
关键词	Genetic transformation Isopentenyl transferase gene (ipt) Petunia Race Senescence Tail-pcr
ISSN号	1532-1819
DOI	10.1080/15321810902782913
通讯作者	Wang, b.(bwang@genetics.ac.cn)
英文摘要	To prevent leaf senescence of petunia, the cytokinin biosynthetic gene isopentenyl transferase (ipt) was placed under the control of 35s promoter and introduced into petunia. pcr analysis showed an expected 0.5 kb fragment of ipt gene in transgenic petunia. rt-pcr analysis indicated the expression of ipt gene in the transgenic lines. leaves from transgenic plants remained green and healthy in normal culture condition, while the non-transformed plants turned to yellow. transgenic plants showed a reduction in height and smaller leaf sizes. in transgenic lines, the internodes were shorter, and the roots grew slower than the non-transformed plants.
WOS关键词	LEAF SENESCENCE ; CYTOKININ
WOS研究方向	Biochemistry & Molecular Biology ; Immunology ; Medical Laboratory Technology
WOS类目	Biochemical Research Methods ; Immunology ; Medical Laboratory Technology
语种	英语
出版者	TAYLOR & FRANCIS INC
WOS记录号	WOS:000264660100009
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2394560
专题	中国科学院大学
通讯作者	Wang, B.
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Bai, L. J.,Ye, C. J.,Lu, J. Y.,et al. Ipt gene transformation in petunia by an agrobacterium mediated method[J]. Journal of immunoassay & immunochemistry,2009,30(2):224-231.
APA	Bai, L. J..,Ye, C. J..,Lu, J. Y..,Yang, D. E..,Xue, H..,...&Liu, M..(2009).Ipt gene transformation in petunia by an agrobacterium mediated method.Journal of immunoassay & immunochemistry,30(2),224-231.
MLA	Bai, L. J.,et al."Ipt gene transformation in petunia by an agrobacterium mediated method".Journal of immunoassay & immunochemistry 30.2(2009):224-231.

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