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	The cation-efflux transporter bjcet2 mediates zinc and cadmium accumulation in brassica juncea l. leaves
	Xu, Jin1; Chai, Tuanyao1; Zhang, Yuxiu2; Lang, Minglin1; Han, Lu1
刊名	Plant cell reports
	2009-08-01
卷号	28期号:8页码:1235-1242
关键词	Heavy metal Cation-efflux transporter Brassica juncea l. Transgenic plant
ISSN号	0721-7714
DOI	10.1007/s00299-009-0723-1
通讯作者	Chai, tuanyao(tychai@gucas.ac.cn)
英文摘要	Brassica juncea l. is a zn/cd accumulator. to determine the physiological basis of its metal accumulation phenotype, the functional properties and role of the metal efflux transporter bjcet2 were investigated using transgenic technology. heterologous expression of bjcet2 in the double mutant yeast strain delta zrc1 delta cot1 enhanced the metal tolerance of the yeast strain and led to decrease in zn or cd accumulation. detection of green fluorescence from green fluorescent protein (gfp) in the root tip of transgenic tobacco further revealed that bjcet2::gfp is localized at the plasma membrane. semi-quantitative rt-pcr analysis showed that bjcet2 was most abundant in the root and was weakly expressed in the stem and leaves. the expression of bjcet2 was up-regulated by heavy metals. however, exposure to low temperature, salt and drought did not affect the expression of bjcet2. overexpression of bjcet2 in transgenic b. juncea plants conferred heavy metal tolerance and increased cd/zn accumulation in the leaves. bjcet2-deficient b. juncea mediated by antisense rna resulted in hypersensitivity to heavy metals and decreased zn/cd accumulation in the plants. these results suggest that the heavy metal efflux of bjcet2 plays important roles in the metal tolerance of b. juncea and in zn/cd accumulation in b. juncea.
WOS关键词	SACCHAROMYCES-CEREVISIAE ; MEMBRANE ; TOBACCO ; ZN
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000268500400008
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2394389
专题	中国科学院大学
通讯作者	Chai, Tuanyao
作者单位	1.Chinese Acad Sci, Coll Life Sci, Grad Univ, Beijing 100049, Peoples R China 2.China Univ Min & Technol Beijing, Dept Bioengn, Sch Chem & Environm Engn, Beijing 100083, Peoples R China
推荐引用方式 GB/T 7714	Xu, Jin,Chai, Tuanyao,Zhang, Yuxiu,et al. The cation-efflux transporter bjcet2 mediates zinc and cadmium accumulation in brassica juncea l. leaves[J]. Plant cell reports,2009,28(8):1235-1242.
APA	Xu, Jin,Chai, Tuanyao,Zhang, Yuxiu,Lang, Minglin,&Han, Lu.(2009).The cation-efflux transporter bjcet2 mediates zinc and cadmium accumulation in brassica juncea l. leaves.Plant cell reports,28(8),1235-1242.
MLA	Xu, Jin,et al."The cation-efflux transporter bjcet2 mediates zinc and cadmium accumulation in brassica juncea l. leaves".Plant cell reports 28.8(2009):1235-1242.

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