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	Identification of a new rice blast resistance gene, pid3, by genomewide comparison of paired nucleotide-binding site-leucine-rich repeat genes and their pseudogene alleles between the two sequenced rice genomes
	Shang, Junjun1,2,4; Tao, Yong1,2,4; Chen, Xuewei1,2,4; Zou, Yan1,2,4; Lei, Cailin3; Wang, Jing1,2,4; Li, Xiaobing1,2; Zhao, Xianfeng1,2; Zhang, Meijun5; Lu, Zhike5
刊名	Genetics
	2009-08-01
卷号	182期号:4页码:1303-1311
ISSN号	0016-6731
DOI	10.1534/genetics.108.102871
通讯作者	Zhu, lihuang(lhzhu@genetics.ac.cn)
英文摘要	Rice blast, caused by magnaporthe oryzae, is one of the most devastating diseases. the two major subspecies of asian cultivated rice (oryza sativa l.), indica and japonica, have shown obvious differences in rice blast resistance but the genomic basis that underlies the difference is not clear. we performed a genomewide comparison of the major class of resistant gene family the nucleotide-binding site-leucine-rich repeat (nbs-lrr) gene family, between 93-11 (indica) and nipponbare (japonica) with a focus on their pseudogene members. we found great differences in either constitution or distribution of pseudogenes between the two genomes. according to this comparison, we designed the pcr-based molecular markers specific to the nipponbare nbs-lrr pseudogene alleles and used them as cosegregation markers for blast susceptibility in a segregation population from a cross between a rice blast-resistant indica variety and a susceptible japonica variety. through this approach, we identified a new blast resistance gene, pid3, in the indica variety, digu. the allelic pid3 loci in most of the tested japonica varieties were identified as pseudogenes due to a nonsense mutation at the nucleotide position 2208 starting from the translation initiation site. however, this mutation was not found in any of the tested indica varieties, african cultivated rice varieties, or aa genome-containing wild rice species. these results suggest that the pseudogenization of pid3 in japonica occurred after the divergence of indica and japonica.
WOS关键词	ORYZA-SATIVA L ; MAGNAPORTHE-GRISEA ; DRAFT SEQUENCE ; JAPONICA RICE ; PROTEIN ; ARABIDOPSIS ; CULTIVATION ; INHERITANCE ; DIVERSITY ; VARIETY
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
语种	英语
出版者	GENETICS
WOS记录号	WOS:000270214000030
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2394327
专题	中国科学院大学
通讯作者	Zhu, Lihuang
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Plant Gene Res Ctr Beijing, Beijing 100101, Peoples R China 3.Chinese Acad Agr Sci, Inst Crop Res, Beijing 100081, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100081, Peoples R China 5.Chinese Acad Sci, Beijing Inst Genom, Beijing 101300, Peoples R China
推荐引用方式 GB/T 7714	Shang, Junjun,Tao, Yong,Chen, Xuewei,et al. Identification of a new rice blast resistance gene, pid3, by genomewide comparison of paired nucleotide-binding site-leucine-rich repeat genes and their pseudogene alleles between the two sequenced rice genomes[J]. Genetics,2009,182(4):1303-1311.
APA	Shang, Junjun.,Tao, Yong.,Chen, Xuewei.,Zou, Yan.,Lei, Cailin.,...&Zhu, Lihuang.(2009).Identification of a new rice blast resistance gene, pid3, by genomewide comparison of paired nucleotide-binding site-leucine-rich repeat genes and their pseudogene alleles between the two sequenced rice genomes.Genetics,182(4),1303-1311.
MLA	Shang, Junjun,et al."Identification of a new rice blast resistance gene, pid3, by genomewide comparison of paired nucleotide-binding site-leucine-rich repeat genes and their pseudogene alleles between the two sequenced rice genomes".Genetics 182.4(2009):1303-1311.

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