Recent advances in proteolysis and peptide/protein separation by chromatographic strategies

	Recent advances in proteolysis and peptide/protein separation by chromatographic strategies
	Zhang XiangMin 1,2; Liu BaoHong 1,2; Zhang LiHua 3; Zou HanFa 3; Cao Jing 1,2; Gao MingXia 1,2; Tang Jia 1,2; Liu Yun 1,2; Yang PengYuan 1,2; Zhang YuKui 3
刊名	science china-chemistry
	2010-04-01
卷号	53 期号:4 页码:685-694
关键词	proteolysis enzymatic digestion chromatographic separation peptide enrichment mass spectrometry
通讯作者	刘云 ; 张玉奎
产权排序	3;2
英文摘要	this review gives a broad glance on the progress of recent advances on proteolysis and peptide/protein separation by chromatographic strategies in the past ten years, covering the main research in these areas especially in china. the reviewed research focused on enzymatic micro-reactors and peptide separation in bottom-up approaches, and protein and peptide separation in top-down approaches. the new enzymatic micro-reactor is able to accelerate proteolytic reaction rate from conventionally a couple of hours to a few seconds, and the multiple dimensional chromatographic-separation with various models or arrays could sufficiently separate the proteomic mixture. these advances have significantly promoted the research of protein/peptide separation and identification in proteomics.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, multidisciplinary
研究领域[WOS]	chemistry
关键词[WOS]	tandem mass-spectrometry ; multidimensional liquid-chromatography ; highly efficient proteolysis ; monolithic enzymatic microreactor ; immobilized trypsin microreactor ; peptide-mapping analysis ; exchange trap column ; lc-esi-ms/ms ; proteome analysis ; human plasma
收录类别	SCI
原文出处	false
语种	英语
WOS记录号	WOS:000278263400002
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/103569]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China 2.Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Zhang XiangMin,Liu BaoHong,Zhang LiHua,et al. Recent advances in proteolysis and peptide/protein separation by chromatographic strategies[J]. science china-chemistry,2010,53(4):685-694.
APA	Zhang XiangMin.,Liu BaoHong.,Zhang LiHua.,Zou HanFa.,Cao Jing.,...&Zhang YuKui.(2010).Recent advances in proteolysis and peptide/protein separation by chromatographic strategies.science china-chemistry,53(4),685-694.
MLA	Zhang XiangMin,et al."Recent advances in proteolysis and peptide/protein separation by chromatographic strategies".science china-chemistry 53.4(2010):685-694.