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	Molecular identification and functional characterization of a drosophila dual-specificity phosphatase dmkp-4 which is involved in pgn-induced activation of the jnk pathway
	Sun, Lei1,2,3; Yu, Ming-Can1,2; Kong, Ling1,2,3; Zhuang, Zi-Heng1,2,3; Hu, Jun-Hao1,2,3; Ge, Bao-Xue1,2
刊名	Cellular signalling
	2008-07-01
卷号	20期号:7页码:1329-1337
关键词	Mapk phosphatases (mkps) Dmkp-4 Map (mitogen-activated protein) kinases Djnk
ISSN号	0898-6568
通讯作者	Ge, bao-xue(gebaoxue@sibs.ac.cn)
英文摘要	Map (mitogen-activated protein) kinases play an important role in regulating many critical cellular processes. the inactivation of map kinases is always accomplished by a family of dual-specificity phosphatases, termed mapk phosphatases (mkps). here, we have identified a novel mkp-like protein, designated dmkp-4, from the drosophila genome. dmkp-4 is a protein of 387 amino acids, with a dual-specificity phosphatase (dsp) catalytic domain. recombinant protein dmkp-4 retains intrinsic phosphatase activity against chromogenic substrate pnpp. overexpression of dmkp-4 inhibited the activation of erk, jnk and p38 by h2o2, sorbitol and heat shock in hek293-t cells, and jnk activation in drosophila s2* cells under pgn stimuli. "knockdown" of dmkp-4 expression by rnai significantly enhanced the pcn-stimulated activation of jnk, but not erk nor p38. further study revealed that dmkp-4 interacted specifically with jnk via its dsp domain. mutation of cys-126 to serine in the dsp domain of dmkp-4 not only eliminated its interaction with jnk, but also markedly reduced its phosphatase activity. thus, dmkp-4 is a drosophila homologue of mammalian mkps, and may play important roles in the regulation of various developmental processes. (c) 2008 elsevier inc. all rights reserved.
WOS关键词	PROTEIN-KINASE PHOSPHATASE-3 ; SIGNAL-REGULATED KINASE ; MAPK PHOSPHATASE ; IMMUNE-RESPONSE ; PEPTIDOGLYCAN RECOGNITION ; CATALYTIC ACTIVATION ; IMD-PATHWAY ; P38 ; BINDING ; GENE
WOS研究方向	Cell Biology
WOS类目	Cell Biology
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000257013000012
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2388096
专题	中国科学院大学
通讯作者	Ge, Bao-Xue
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Hlth Sci, Lab Signal Transduct, Shanghai 200025, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Med, Shanghai 200025, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China
推荐引用方式 GB/T 7714	Sun, Lei,Yu, Ming-Can,Kong, Ling,et al. Molecular identification and functional characterization of a drosophila dual-specificity phosphatase dmkp-4 which is involved in pgn-induced activation of the jnk pathway[J]. Cellular signalling,2008,20(7):1329-1337.
APA	Sun, Lei,Yu, Ming-Can,Kong, Ling,Zhuang, Zi-Heng,Hu, Jun-Hao,&Ge, Bao-Xue.(2008).Molecular identification and functional characterization of a drosophila dual-specificity phosphatase dmkp-4 which is involved in pgn-induced activation of the jnk pathway.Cellular signalling,20(7),1329-1337.
MLA	Sun, Lei,et al."Molecular identification and functional characterization of a drosophila dual-specificity phosphatase dmkp-4 which is involved in pgn-induced activation of the jnk pathway".Cellular signalling 20.7(2008):1329-1337.

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