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	The differentiation of rat adipose-derived stem cells into oec-like cells on collagen scaffolds by co-culturing with oecs
	Wang, Bin; Han, Jin; Gao, Yuan; Xiao, Zhifeng; Chen, Bing; Wang, Xia; Zhao, Wenxue; Dai, Jianwu
刊名	Neuroscience letters
	2007-06-29
卷号	421期号:3页码:191-196
关键词	Oecs Adscs Differentiation Co-culture Three-dimensional culture
ISSN号	0304-3940
DOI	10.1016/j.neulet.2007.04.081
通讯作者	Dai, jianwu(jwdai@genetics.ac.cn)
英文摘要	Olfactory ensheathing cells (oecs) transplantation is a promising or potential therapy for spinal cord injury (sci). however, their clinical use is limited because of the availability. adipose-derived stem cells (adscs) have been identified as an alternative source of adult stein cells in recent years. adscs could be differentiated into various mesenchymal tissues cells such as chondrocytes, adipocytes, osteoblasts, and myocytes and also could be differentiated into neural lineages. in this study, we examined the feasibility of using adscs as a source of stem cells for the differentiation of oecs by co-culture approach. when co-cultured with oecs, the adscs on three-dimensional collagen scaffolds were differentiated into oec-like cells, with similar morphology and antigenic phenotypes (p75ntr+/nestin+/gfap-) of oecs. co-cultured adscs were positive for several important functional markers of mature oecs such as neurotrophic factor gdnf, bdnf and myelin protein plp and the conditioned medium of ofc-like cells could significantly promote drg neuron growth and axon sprouting without ngf supporting in contrast to that of the adscs. our results showed that adscs had the potential to differentiate into oec-like cells on the three-dimensional collagen scaffolds in vitro. (c) 2007 published by elsevier ireland ltd.
WOS关键词	OLFACTORY ENSHEATHING CELLS ; SPINAL-CORD ; SCHWANN-CELLS ; IN-VITRO ; STROMAL CELLS ; ADULT CNS ; TRANSPLANTATION ; TISSUE ; REGENERATION ; EXPRESSION
WOS研究方向	Neurosciences & Neurology
WOS类目	Neurosciences
语种	英语
出版者	ELSEVIER IRELAND LTD
WOS记录号	WOS:000248152100002
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2383208
专题	中国科学院大学
通讯作者	Dai, Jianwu
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Mol Dev Biol, Beijing 100864, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China
推荐引用方式 GB/T 7714	Wang, Bin,Han, Jin,Gao, Yuan,et al. The differentiation of rat adipose-derived stem cells into oec-like cells on collagen scaffolds by co-culturing with oecs[J]. Neuroscience letters,2007,421(3):191-196.
APA	Wang, Bin.,Han, Jin.,Gao, Yuan.,Xiao, Zhifeng.,Chen, Bing.,...&Dai, Jianwu.(2007).The differentiation of rat adipose-derived stem cells into oec-like cells on collagen scaffolds by co-culturing with oecs.Neuroscience letters,421(3),191-196.
MLA	Wang, Bin,et al."The differentiation of rat adipose-derived stem cells into oec-like cells on collagen scaffolds by co-culturing with oecs".Neuroscience letters 421.3(2007):191-196.

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