| Fast and accurate measurement of diffusion coefficient by taylor's dispersion analysis | |
| Zuo Min1,2,3; Han Yanli1,2,3; Qi Li1,2; Chen Yi1,2 | |
| 刊名 | Chinese science bulletin
 |
| 2007-12-01 | |
| 卷号 | 52期号:24页码:3325-3332 |
| 关键词 | Diffusion coefficient Aqueous and nonaqueous samples Taylor's dispersion analysis Capillary electrophoresis Fast and accurate measurement |
| ISSN号 | 1001-6538 |
| DOI | 10.1007/s11434-007-0473-9 |
| 通讯作者 | Chen yi(chenyi@iccas.ac.cn) |
| 英文摘要 | This investigation aimed at improving the performance of taylor's dispersion analysis for the fast and accurate measurement of diffusion coefficient of a minute solute in various solvents. the investigation was carried out on a capillary electrophoresis instrument by monitoring the uv absorption peak of a solute pulse and calculating the diffusion coefficient by peak efficiency. with l-phenylalanine as a main testing solute, some key factors were afterward disclosed including especially the capillary size, carrier flow velocity, injection volume and capillary conditioning. peak tailing, large volume of sample injection and slow migration were found to underestimate the diffusion coefficient while very fast migration and high sample concentration caused overestimation. at a moderate flow velocity of 0.1-1 cm/s with a capillary of 72.44 mu m i.d. x60 cm (50 cm effective) maintained at 25 degrees c, the diffusion coefficient of aqueous l-phenylalanine was determined, giving a value of 7.02x10(-6) cm(2)/s with error < 2% and relative standard deviation < 0.2% (n=3). the method was shown to be applicable to the measurement of various samples such as aqueous phenylalanine, acetone, phenol, toluene and benzene, and nonaqueous benzene (in ethanol or 1-butanol). |
| WOS关键词 | SPIN-ECHO NMR ; LIGHT-SCATTERING ; AQUEOUS-SOLUTIONS ; SELF-DIFFUSION ; DIAPHRAGM CELL ; GRADIENT ; CAPILLARIES ; WATER ; MIXTURES ; PROTEINS |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| 出版者 | SCIENCE CHINA PRESS |
| WOS记录号 | WOS:000252170700003 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2381752 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chen Yi |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Inst Chem, Lab Analyt Chem & Life Sci, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zuo Min,Han Yanli,Qi Li,et al. Fast and accurate measurement of diffusion coefficient by taylor's dispersion analysis[J]. Chinese science bulletin,2007,52(24):3325-3332. |
| APA | Zuo Min,Han Yanli,Qi Li,&Chen Yi.(2007).Fast and accurate measurement of diffusion coefficient by taylor's dispersion analysis.Chinese science bulletin,52(24),3325-3332. |
| MLA | Zuo Min,et al."Fast and accurate measurement of diffusion coefficient by taylor's dispersion analysis".Chinese science bulletin 52.24(2007):3325-3332. |
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