Green and orange cdte quantum dots as effective ph-sensitive fluorescent probes for dual simultaneous and independent detection of viruses | |
Deng, Zhengtao; Zhang, Yun; Yue, Jiachang; Tang, Fangqiong; Wei, Qun | |
刊名 | Journal of physical chemistry b |
2007-10-18 | |
卷号 | 111期号:41页码:12024-12031 |
ISSN号 | 1520-6106 |
DOI | 10.1021/jp074609z |
通讯作者 | Yue, jiachang(yuejc@sun5.ibp.ac.cn) |
英文摘要 | One of the most highlighted and fastest moving interfaces of nanotechnology is the application of quantum dots (qds) in biology. the unparalleled advantages of the size-tunable fluorescent emission and the simultaneous excitation at a single wavelength make qds the great possibility for use in optical encoding detection. in this paper, we report that green and orange cdte qds as convenient, cheap, reversible, and effective ph-sensitive fluorescent probes could monitor the proton (h+) flux driven by atp synthesis for dual simultaneous and independent detection of viruses on the basis of antibody-antigen reactions. a new kind of biosensor (consisting of the mixture of green-qds-labeled chromatophores and orange-qds-labeled chromatophores) fluorescent measurement system was established for rapid, simultaneous, and independent detection of two different kinds of viruses (i.e., h9 avian influenza virus and mhv68 virus). it is crucial to find that the green and orange qds labeled biosensors, coexisting in the detection system can work independently and do not interfere with each another in the fluorescence assays. in addition, a primary steady electric double layer (edl) model for the qds biosensors was proposed to illustrate the mechanism of simultaneous and independent detection of the biosensors. we believe that the ph-sensitive cdte qds based detection system, described in this paper, is an important step toward optical encoding and has a great potential for simultaneous and independent qualitative and quantitative multiple detection systems. |
WOS关键词 | RHODOSPIRILLUM-RUBRUM ; MOLECULAR MACHINE ; NANOCRYSTALS ; CDSE ; LUMINESCENT ; F0F1-ATPASE ; QUALITY ; SENSOR ; ROUTE |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Physical |
语种 | 英语 |
出版者 | AMER CHEMICAL SOC |
WOS记录号 | WOS:000250142400025 |
内容类型 | 期刊论文 |
URI标识 | http://www.corc.org.cn/handle/1471x/2381432 |
专题 | 中国科学院大学 |
通讯作者 | Yue, Jiachang |
作者单位 | 1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Tech Inst Phys & Chem, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China 4.Xian Jiaotong Univ, Sch Life Sci & Technol, Xian 710049, Peoples R China 5.Beijing Normal Univ, Dept Biochem & Mol Biol, Beijing Keb Lab, Beijing 100875, Peoples R China |
推荐引用方式 GB/T 7714 | Deng, Zhengtao,Zhang, Yun,Yue, Jiachang,et al. Green and orange cdte quantum dots as effective ph-sensitive fluorescent probes for dual simultaneous and independent detection of viruses[J]. Journal of physical chemistry b,2007,111(41):12024-12031. |
APA | Deng, Zhengtao,Zhang, Yun,Yue, Jiachang,Tang, Fangqiong,&Wei, Qun.(2007).Green and orange cdte quantum dots as effective ph-sensitive fluorescent probes for dual simultaneous and independent detection of viruses.Journal of physical chemistry b,111(41),12024-12031. |
MLA | Deng, Zhengtao,et al."Green and orange cdte quantum dots as effective ph-sensitive fluorescent probes for dual simultaneous and independent detection of viruses".Journal of physical chemistry b 111.41(2007):12024-12031. |
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