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Green and orange cdte quantum dots as effective ph-sensitive fluorescent probes for dual simultaneous and independent detection of viruses
Deng, Zhengtao; Zhang, Yun; Yue, Jiachang; Tang, Fangqiong; Wei, Qun
刊名Journal of physical chemistry b
2007-10-18
卷号111期号:41页码:12024-12031
ISSN号1520-6106
DOI10.1021/jp074609z
通讯作者Yue, jiachang(yuejc@sun5.ibp.ac.cn)
英文摘要One of the most highlighted and fastest moving interfaces of nanotechnology is the application of quantum dots (qds) in biology. the unparalleled advantages of the size-tunable fluorescent emission and the simultaneous excitation at a single wavelength make qds the great possibility for use in optical encoding detection. in this paper, we report that green and orange cdte qds as convenient, cheap, reversible, and effective ph-sensitive fluorescent probes could monitor the proton (h+) flux driven by atp synthesis for dual simultaneous and independent detection of viruses on the basis of antibody-antigen reactions. a new kind of biosensor (consisting of the mixture of green-qds-labeled chromatophores and orange-qds-labeled chromatophores) fluorescent measurement system was established for rapid, simultaneous, and independent detection of two different kinds of viruses (i.e., h9 avian influenza virus and mhv68 virus). it is crucial to find that the green and orange qds labeled biosensors, coexisting in the detection system can work independently and do not interfere with each another in the fluorescence assays. in addition, a primary steady electric double layer (edl) model for the qds biosensors was proposed to illustrate the mechanism of simultaneous and independent detection of the biosensors. we believe that the ph-sensitive cdte qds based detection system, described in this paper, is an important step toward optical encoding and has a great potential for simultaneous and independent qualitative and quantitative multiple detection systems.
WOS关键词RHODOSPIRILLUM-RUBRUM ; MOLECULAR MACHINE ; NANOCRYSTALS ; CDSE ; LUMINESCENT ; F0F1-ATPASE ; QUALITY ; SENSOR ; ROUTE
WOS研究方向Chemistry
WOS类目Chemistry, Physical
语种英语
出版者AMER CHEMICAL SOC
WOS记录号WOS:000250142400025
内容类型期刊论文
URI标识http://www.corc.org.cn/handle/1471x/2381432
专题中国科学院大学
通讯作者Yue, Jiachang
作者单位1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Tech Inst Phys & Chem, Beijing 100080, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China
4.Xian Jiaotong Univ, Sch Life Sci & Technol, Xian 710049, Peoples R China
5.Beijing Normal Univ, Dept Biochem & Mol Biol, Beijing Keb Lab, Beijing 100875, Peoples R China
推荐引用方式
GB/T 7714
Deng, Zhengtao,Zhang, Yun,Yue, Jiachang,et al. Green and orange cdte quantum dots as effective ph-sensitive fluorescent probes for dual simultaneous and independent detection of viruses[J]. Journal of physical chemistry b,2007,111(41):12024-12031.
APA Deng, Zhengtao,Zhang, Yun,Yue, Jiachang,Tang, Fangqiong,&Wei, Qun.(2007).Green and orange cdte quantum dots as effective ph-sensitive fluorescent probes for dual simultaneous and independent detection of viruses.Journal of physical chemistry b,111(41),12024-12031.
MLA Deng, Zhengtao,et al."Green and orange cdte quantum dots as effective ph-sensitive fluorescent probes for dual simultaneous and independent detection of viruses".Journal of physical chemistry b 111.41(2007):12024-12031.
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